Pawłowski Piotr H
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, A. Pawińskiego 5a, Warsaw, Poland.
Acta Biochim Pol. 2009;56(3):471-80. Epub 2009 Sep 14.
A simple theoretical model considering cell membrane mechanosensitivity can accurately describe published experimental data on membrane area creeping and recovery, and on osmotic expansion and rupture. The model to data fit reveals real values of membrane tension and elasticity modulus, and the parameters describing membrane organization and kinetics of mechanosensitive membrane traffic, including small solute transport, water permeability, endocytosis, exocytosis, and caveolae formation. This estimation allows for separation and quantitative analysis of the participation of different processes constituting the response of plasmalemma to short time-scale membrane load. The predicted properties of the model were verified for membrane stretching at different osmotic pressures. Finally, a simple hypothesis concerning stressed cell membrane breakdown is postulated.
一个考虑细胞膜机械敏感性的简单理论模型能够准确描述已发表的关于膜面积蠕变与恢复、渗透膨胀与破裂的实验数据。模型与数据的拟合揭示了膜张力和弹性模量的实际值，以及描述膜组织和机械敏感膜运输动力学的参数，包括小分子溶质运输、水渗透性、内吞作用、外排作用和小窝形成。这种估计允许对构成质膜对短时间尺度膜负荷响应的不同过程的参与进行分离和定量分析。该模型预测的特性在不同渗透压下的膜拉伸实验中得到了验证。最后，提出了一个关于应激细胞膜破裂的简单假说。
