Research Laboratory, National Atomic Energy Commission CNEA-Regional Noroeste, Argentina National Research Council CONICET, A4408FTV, Salta C1033AAJ, Argentina.
Biol Trace Elem Res. 2010 Jun;135(1-3):242-52. doi: 10.1007/s12011-009-8512-9. Epub 2009 Sep 15.
Few reports are available in the literature on enamel formation under nutritional deficiencies. Thus, we performed a study to determine the effects of boron (B) deficiency on the maturing dental enamel, employing the rat continuously erupting incisor as the experimental model. Male Wistar rats, 21 days old, were used throughout. They were divided into two groups, each containing ten animals: +B (adequate; 3-mg B/kg diet) and -B (boron deficient; 0.07-mg B/kg diet). The animals were maintained on their respective diets for 14 days and then euthanized. The mandibles were resected, fixed, and processed for embedding in paraffin and/or methyl methacrylate. Oriented histological sections of the continuously erupting incisor were obtained at the level of the mesial root of the first molar, allowing access to the maturation zone of the developing enamel. Dietary treatment did not affect food intake and body weight. Histomorphometric evaluation using undecalcified sections showed a reduction in enamel thickness (hypoplasia), whereas microchemical characterization by energy-dispersive X-ray spectrometry did not reveal alterations in enamel mineralization.
关于营养缺乏条件下釉质形成的文献报道较少。因此，我们采用大鼠不断萌出切牙作为实验模型，进行了一项研究，以确定硼（B）缺乏对成熟牙釉质的影响。雄性 Wistar 大鼠，21 日龄，用于整个研究。将它们分为两组，每组包含 10 只动物：+B（充足；3mg/kg 饮食中的 B）和-B（硼缺乏；0.07mg/kg 饮食中的 B）。动物在各自的饮食中维持 14 天，然后安乐死。切除下颌骨，固定，然后进行石蜡和/或甲基丙烯酸甲酯包埋处理。在第一磨牙近中根的水平上获得连续萌出切牙的定向组织学切片，可获得发育中牙釉质的成熟区。饮食处理不影响食物摄入和体重。未脱钙切片的组织形态计量学评估显示釉质厚度减少（发育不全），而能量色散 X 射线光谱学的微观化学特征并未显示牙釉质矿化的改变。
