2nd Department of Medicine and Nephrological Center, Faculty of Medicine, University of Pécs, Pécs, Hungary.
Physiol Meas. 2009 Oct;30(10):1137-50. doi: 10.1088/0967-3334/30/10/012. Epub 2009 Sep 16.
The measurement of the excretion of urinary albumin (albuminuria) is an important and well-established method to assess clinical outcomes. A high-performance liquid chromatography (HPLC) method has been introduced to measure albuminuria. Using this method, it was found that commonly used immunological methods do not measure a fraction of urinary albumin. Some authors presumed that the reason of immuno-unreactivity is the modification of urinary albumin; some others presumed that the difference is merely because of interference. In order to decide this question, we established an HPLC method equipped with tandem UV and fluorescent detection to assess the changes in the detectability of albumin with the rate of modification. For this measurement, differently modified forms of albumin were used. Urine samples of diabetic patients were also measured to find a potential connection between the modification rate and clinical parameters. Secondly, we have established a reversed phase HPLC method to assess the interference rate. We conclude that albumin modification does not affect immunoreactivity. The modification rate of urinary albumin in diabetic patients showed a correlation with renal function. The interference rate of the albumin peak was found to be 12.7% on average, which does not explain the difference between the two methods.
尿白蛋白（白蛋白尿）排泄的测量是评估临床结果的一种重要且成熟的方法。已经引入了一种高效液相色谱（HPLC）方法来测量白蛋白尿。使用该方法，发现常用的免疫学方法不能测量尿液白蛋白的一部分。一些作者推测免疫反应性差的原因是尿液白蛋白的修饰；另一些作者则推测差异仅仅是因为干扰。为了解决这个问题，我们建立了一种配备串联紫外和荧光检测的 HPLC 方法，以评估白蛋白检测率随修饰率的变化。为此，我们使用了不同修饰形式的白蛋白。还测量了糖尿病患者的尿液样本，以发现修饰率与临床参数之间的潜在联系。其次，我们建立了反相 HPLC 方法来评估干扰率。我们的结论是白蛋白修饰不会影响免疫反应性。糖尿病患者尿白蛋白的修饰率与肾功能有关。白蛋白峰的干扰率平均为 12.7%，这不能解释两种方法之间的差异。
