Institute of Genetics and Biophysics Adriano Buzzati-Traverso, Consiglio Nazionale delle Ricerche, Via Pietro Castellino, 111, 80131 Naples, Italy.
Biometals. 2009 Dec;22(6):1089-94. doi: 10.1007/s10534-009-9259-7. Epub 2009 Sep 4.
The effects of potassium tellurite on growth and survival of rho(+) and rho(0) Saccharomyces cerevisiae strains were investigated. Both rho(+) and rho(0) strains grew on a fermentable carbon source with up to 1.2 mM K(2)TeO(3), while rho(+) yeast cells grown on a non-fermentable carbon source were inhibited at tellurite levels as low as 50 muM suggesting that this metalloid specifically inhibited mitochondrial functions. Growth of rho(+) yeast cells in the presence of increasing amount of tellurite resulted in dose-dependent blackening of the culture, a phenomenon not observed with rho(0) cultures. Transmission electron microscopy of S. cerevisiae rho(+) cells grown in the presence of tellurite showed that blackening was likely due to elemental tellurium (Te(0)) that formed large deposits along the cell wall and small precipitates in both the cytoplasm and mitochondria.
研究了碲化钾对 rho(+)和 rho(0)酿酒酵母菌株生长和存活的影响。rho(+)和 rho(0)菌株都能在高达 1.2 mM K(2)TeO(3)的可发酵碳源上生长，而在非发酵碳源上生长的 rho(+)酵母细胞在低至 50 μM 的碲化物水平下受到抑制，表明这种类金属特别抑制了线粒体功能。在存在越来越多的碲化物的情况下，rho(+)酵母细胞的生长导致培养物的剂量依赖性变黑，而 rho(0)培养物则没有观察到这种现象。在碲化物存在下生长的酿酒酵母 rho(+)细胞的透射电子显微镜显示，黑化可能是由于元素碲 (Te(0))形成的，这些碲沿着细胞壁形成大的沉积物，在细胞质和线粒体中形成小的沉淀物。
