Université de Toulouse, INSA, UPS, INP, LISBP, 135 Av. de Rangueil, F-31077 Toulouse, France.
Bioresour Technol. 2011 Jan;102(2):1521-7. doi: 10.1016/j.biortech.2010.08.003. Epub 2010 Aug 13.
Glycerol is an agro-industrial residue generated in high amounts during the biodiesel production. The growing production of biodiesel is creating a worldwide glycerol surplus. Therefore, replacing sugar-based feedstock in bioprocesses by glycerol could be potentially attractive. Saccharomyces cerevisiae is one of the most commonly used microorganisms in the agri-food industry and therefore currently produced in large quantities from sugar-based feedstock. Unfortunately, growth of S. cerevisiae strains on glycerol is very low with reported μmax around 0.01 h(-1). This study demonstrates that successive growth of the S. cerevisiae CBS 8066, CEN.PK 113-7 D and Ethanol Red on glycerol as sole carbon source considerably improved the μmax from 0.01 up to 0.2 h(-1). The "adapted strain" CBS 8066-FL20 was kinetically characterized during aerobic and oxygen-limited cultivation in bioreactor and the results discussed in terms of their implication for developing glycerol-based S. cerevisiae bioprocesses.
甘油是生物柴油生产过程中大量产生的农业工业副产物。生物柴油产量的不断增加正在导致全球甘油过剩。因此，用甘油替代生物工艺中的糖基原料可能具有吸引力。酿酒酵母是农业食品工业中最常用的微生物之一，因此目前大量使用糖基原料生产。不幸的是，据报道，甘油上酿酒酵母菌株的生长非常缓慢，μmax 约为 0.01 h(-1)。本研究表明，连续生长的酿酒酵母 CBS 8066、CEN.PK 113-7 D 和 Ethanol Red 在甘油作为唯一碳源时，μmax 从 0.01 提高到 0.2 h(-1)。“适应株”CBS 8066-FL20 在生物反应器中的好氧和缺氧培养过程中的动力学特性进行了表征，并根据其对开发基于甘油的酿酒酵母生物工艺的意义进行了讨论。
