Zhu Yi, Bai Chun-yan, Zhuo Lian-shi, Feng Wen-qi
College of Chinese Medicine, Chongqing Medical University, Chongqing 401331, China.
Zhen Ci Yan Jiu. 2009 Jun;34(3):175-9.
To study the underlying mechanism of electroacupuncture (EA) in relieving depression.
A total of 30 Wistar rats were randomly divided into normal control group (n=10), model group (n=10) and EA group (n=10). Depression model was established by lonely raising and chronic unpredictable mild stress for 21 days. EA (2 Hz, 1 mA) was applied to "Baihui" (GV 20) and "Sanyinjiao" (SP 6) for 20 min, once daily for 14 days. The expression (option density) of hippocampal somatostatin (SS) and SS mRNA was detected by using immunohistochemistry and reversed transcription-polymerase chain reaction (RT-PCR) techniques respectively.
Compared to normal control group, the crossing movement and rearing movement times of open-field test, and sucrose consumption in model group decreased significantly (P < 0.01), and hippocampal SS and SS mRNA expressions were downregulated remarkably (P < 0.05). Compared to model group, the crossing movement and rearing movement times in EA group increased considerably (P < 0.01), and hippocampal SS and SS mRNA expressions were upregulated remarkably (P < 0.05). No significant difference was found between EA and normal control groups in SS and SS mRNA expression.
EA can effectively improve depression rats' behavior activity and upregulate hippocampal SS and SS mRNA expression.
研究电针缓解抑郁症的潜在机制。
将30只Wistar大鼠随机分为正常对照组（n = 10）、模型组（n = 10）和电针组（n = 10）。采用孤养结合慢性不可预测性温和应激法建立抑郁症模型，持续21天。将电针（2 Hz，1 mA）施加于“百会”（GV 20）和“三阴交”（SP 6），每次20分钟，每日1次，共14天。分别采用免疫组化和逆转录-聚合酶链反应（RT-PCR）技术检测海马生长抑素（SS）及其mRNA的表达（光密度值）。
与正常对照组相比，模型组旷场试验中的穿越运动和直立运动次数以及蔗糖消耗量显著减少（P < 0.01），海马SS及其mRNA表达明显下调（P < 0.05）。与模型组相比，电针组的穿越运动和直立运动次数显著增加（P < 0.01），海马SS及其mRNA表达明显上调（P < 0.05）。电针组与正常对照组在SS及其mRNA表达方面无显著差异。
电针可有效改善抑郁大鼠的行为活动，并上调海马SS及其mRNA表达。
