Yu Bin, Sun Yong, Lü Gao-Hong, Lu Jin-Fu, Fang Tai-Hui
Key Laboratory of Integrative Acupuncture and Drugs of Jiangsu Province and Education Ministry of PR China, Nanjing University of Chinese Medicine, Nanjing 210046, China.
Zhen Ci Yan Jiu. 2011 Jun;36(3):164-70.
To observe the effect of combined administration of intragastric perfusion of extract of Hypericum Perforatum L (HP-L) and electroacupuncture (EA) of "Baihui" (GV 20) and "Yamen" (GV 15) on behavior and brain microcirculation in depression rats.
Female SD rats were randomized into control, model, lower-dose of HP-L (lower-dose in short, 10 mg/kg), lower-dose+ EA, higher-dose (20 mg/kg) and higher-dose+ EA groups (n = 10/group). Depression model was established by lonely raising and chronic unpredictable mild stress (tail cramping, water-deprivation, fasting, electrical shock stimulation, etc. ) for 21 days. EA (2 Hz, 1 mA) was applied to "Baihui"(GV 20) and "Yamen"(GV 15) for 20 min, once daily for 14 days. Changes of ethology including glucose-consumption during 1 h, crossing and rearing scores of open-field test during 3 min (for assessing the rats' locomoto)and laser Doppler flowmetry values of cortical regional cerebral bloodflow (r CBF) were detected, and Morris water maze test (for assessing the rats' learning-memory ability) was conducted.
In comparison with the control group, the sucrose consumption, crossing and rearing scores of open-field test, the average swimming velocity (ASV). the ratios of path length and swimming duration near the hidden-platform and the path length and swimming duration far from the platform of Morris water maze test during 70 seconds, and the cortical r CBF value in the model group were decreased significantly (P < 0.01), while the total swimming distance and escape latency in the model group increased apparently (P < 0.01). Compared to the model group, the average sucrose consumption, crossing and rearing scores of open-field test, the ASV, and the ratios of path length and swimming duration near the platform and those far from the platform in the lower-dose. lower-dose + EA, higher-dose and higher-dose + EA groups, and the cortical r CBF in the lower-dose + EA and higher-dose + EA groups were increased considerably (P < 0.05, P < 0.01). The total swimming distances and escape latencies of lower-dose, lower-dose + EA, higher-dose and higher-dose + EA groups were significantly shortened in comparison with the model group (P < 0.05, P < 0.01). The sucrose consumption and crossing score were significantly higher in the higher-dose + EA group than the lower-dose group (P < 0.05). The escape latency was significantly shorter in the higher-dose + EA group than in the lower-dose group (P < 0.05). No significant differences were found among the lower-dose, lower-dose + EA and higher-dose groups the sucrose consumption, crossing score and escape latency: among the lower-dose, lower-dose + EA, higher-dose and higher-dose + EA groups in the rearing score and ASV; among the lower-dose, higher-dose and model groups in the cortical r CBF (P > 0.05).
EA can enhance the effect of extract of HP-L in increasing sucrose consumption, crossing score and cerebral blood flow, and in shortening escape latency in depression rats, which may contribute to their effect in improving depression. But HP-L itself has no effect on cortical microcirculation.
观察贯叶金丝桃提取物（HP-L）灌胃联合电针“百会”（GV 20）、“哑门”（GV 15）对抑郁大鼠行为及脑微循环的影响。
将雌性SD大鼠随机分为对照组、模型组、HP-L低剂量组（简称低剂量组，10 mg/kg）、低剂量+电针组、HP-L高剂量组（20 mg/kg）和高剂量+电针组（每组n = 10）。采用孤养结合慢性不可预知温和应激（尾夹、禁水、禁食、电击刺激等）方法建立抑郁模型，持续21天。电针“百会”（GV 20）、“哑门”（GV 15），频率2 Hz，强度1 mA，每次20分钟，每日1次，共14天。检测行为学变化，包括1小时内的蔗糖消耗量、旷场试验3分钟内的穿越和直立得分（用于评估大鼠运动能力）以及皮质局部脑血流（r CBF）的激光多普勒血流仪值，并进行Morris水迷宫试验（用于评估大鼠学习记忆能力）。
与对照组相比，模型组的蔗糖消耗量、旷场试验穿越和直立得分、平均游泳速度（ASV）、Morris水迷宫试验70秒内隐藏平台附近路径长度与游泳时间的比值、远离平台的路径长度与游泳时间的比值以及皮质r CBF值均显著降低（P < 0.01），而模型组的总游泳距离和逃避潜伏期明显增加（P < 0.01）。与模型组相比，低剂量组、低剂量+电针组、高剂量组和高剂量+电针组的平均蔗糖消耗量、旷场试验穿越和直立得分、ASV、平台附近和远离平台的路径长度与游泳时间的比值以及低剂量+电针组和高剂量+电针组的皮质r CBF均显著增加（P < 0.05，P < 0.01）。低剂量组、低剂量+电针组、高剂量组和高剂量+电针组的总游泳距离和逃避潜伏期与模型组相比均显著缩短（P < 0.05，P < 0.01）。高剂量+电针组的蔗糖消耗量和穿越得分显著高于低剂量组（P < 0.05）。高剂量+电针组的逃避潜伏期显著短于低剂量组（P < 0.05）。低剂量组、低剂量+电针组和高剂量组之间的蔗糖消耗量、穿越得分和逃避潜伏期；低剂量组、低剂量+电针组、高剂量组和高剂量+电针组之间的直立得分和ASV；低剂量组、高剂量组和模型组之间的皮质r CBF比较，差异均无统计学意义（P > 0.05）。
电针可增强HP-L提取物对增加抑郁大鼠蔗糖消耗量、穿越得分和脑血流量以及缩短逃避潜伏期的作用效果，这可能有助于其改善抑郁的作用。但HP-L本身对皮质微循环无影响。
