McReynolds J, Waneck C, Byrd J, Genovese K, Duke S, Nisbet D
USDA-Agricultural Research Service-Southern Plains Agricultural Research Center, College Station, TX 77845, USA.
Poult Sci. 2009 Oct;88(10):2075-80. doi: 10.3382/ps.2009-00106.
Our laboratory is evaluating the efficacy of direct-fed microbials (DFM) and phytogenic products to control Clostridium perfringens, a gram-positive organism associated with decreased performance and morbidity and mortality associated with necrotic enteritis, as well as some recent human food safety issues. Three experiments were conducted to evaluate a DFM (PoultryStar) and a phytogenic product (PEP125), which were administered to birds from day of hatch until termination (d 25) via the drinking water or through supplementation to a wheat-corn diet, respectively. Each experiment contained a nonchallenged negative control and a positive control wherein birds were immunocompromised with a 10x dosage of infectious bursal disease vaccine at 14 d of age and subsequently gavaged with C. perfringens (10(7) cfu/mL) daily for 3 consecutive days starting on d 17. Intestinal lesions, mortality, and log10 values of C. perfringens in the probiotic and phytogenic treatment groups were found to be lower (P<0.05) than those observed in the positive controls. These experiments suggest that the DFM and the phytogenic product could be used as potential alternatives to help control C. perfringens and necrotic enteritis.
我们实验室正在评估直接投喂微生物（DFM）和植物源产品对控制产气荚膜梭菌的效果，产气荚膜梭菌是一种革兰氏阳性菌，与生产性能下降、坏死性肠炎相关的发病率和死亡率有关，还与近期一些人类食品安全问题有关。进行了三项试验来评估一种DFM（PoultryStar）和一种植物源产品（PEP125），分别在雏鸡出壳当天至试验结束（第25天）通过饮水或添加到小麦-玉米日粮中进行投喂。每项试验都设有一个未受攻毒的阴性对照和一个阳性对照，在阳性对照中，雏鸡在14日龄时用10倍剂量的传染性法氏囊病疫苗进行免疫抑制，随后从17日龄开始连续3天每天灌喂产气荚膜梭菌（10⁷ cfu/mL）。结果发现，益生菌和植物源产品处理组的肠道病变、死亡率以及产气荚膜梭菌的log10值均低于阳性对照组（P<0.05）。这些试验表明，DFM和植物源产品可作为控制产气荚膜梭菌和坏死性肠炎的潜在替代物。
