Yan Yajun, Lee Chia-Chi, Liao James C
Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531, Boelter Hall, 420 Westwood Plaza, Los Angeles, CA 90095, USA.
Org Biomol Chem. 2009 Oct 7;7(19):3914-7. doi: 10.1039/b913501d. Epub 2009 Aug 3.
We characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).
我们表征了四种仲醇脱氢酶（sADH）对乙偶姻还原的活性和立体特异性，并在大肠杆菌中构建了合成途径，以从葡萄糖生产对映体纯的（R，R）-2,3-丁二醇（2,3-BDO），其滴度为6.1 g/L（对映体纯度>99%），产物产率为0.31 g产物/g葡萄糖（为理论最大值的62%）。
