基于离子液体的超声辅助提取粉防己碱和汉防己甲素的方法。

Ionic liquid-based ultrasound-assisted extraction of fangchinoline and tetrandrine from Stephaniae tetrandrae.

机构信息

Shandong Analysis and Test Center, Shandong Academy of Sciences, Jinan, Shandong, China.

出版信息

J Sep Sci. 2009 Oct;32(20):3550-4. doi: 10.1002/jssc.200900413.

DOI:10.1002/jssc.200900413

PMID:19764054

Abstract

An ionic liquid-based ultrasound-assisted extraction method has been developed for the effective extraction of fangchinoline and tetrandrine from Stephaniae tetrandrae. The effects of some ultrasound-assisted extraction parameters including the concentration of [BMIM][BF(4)], pH, ultrasonic power and time were investigated to optimize the ultrasound-assisted extraction conditions. Compared to the regular ultrasound-assisted extraction and traditional refluent extraction, the proposed [BMIM][BF(4)]-based ultrasound-assisted extraction offered shorter extraction times (from 6 h to 40 min) and remarkable higher efficiencies (approximately 30% improved), which supported the suitability of the proposed approach. In addition, the proposed approach was confirmed by the good correlation coefficient (R(2)), recovery and reproducibility (RSD, n = 5), which were in the range of 0.9992-0.9995, 85.5-101.1%, and 1.87-4.33%, respectively.

摘要

一种基于离子液体的超声辅助提取方法已被开发用于从防己中有效提取粉防己碱和汉防己甲素。考察了一些超声辅助提取参数的影响，包括[BMIM][BF4]的浓度、pH 值、超声功率和时间，以优化超声辅助提取条件。与常规超声辅助提取和传统回流提取相比，所提出的基于[BMIM][BF4]的超声辅助提取提供了更短的提取时间（从 6 小时到 40 分钟）和显著更高的效率（约提高 30%），这证明了该方法的适用性。此外，所提出的方法通过良好的相关系数（R²）、回收率和重现性（RSD，n=5）得到证实，范围分别为 0.9992-0.9995、85.5-101.1%和 1.87-4.33%。