Gao Wenhua, Lin Shaoyu, Chen Yaowen, Chen Anjia, Li Yuqin, Chen Xingguo, Hu Zhide
Department of Chemistry, Lanzhou University, Lanzhou 730000, PR China.
J Sep Sci. 2005 May;28(7):639-46. doi: 10.1002/jssc.200400034.
A simple, rapid, and sensitive non-aqueous capillary electrophoresis procedure with head-column field-amplified sample stacking concentration for the analysis of fangchinoline and tetrandrine is established. Optimum separation and stacking conditions were obtained when the sample was injected at 8 kV for 50 s after preliminary pressure injection of ethanol (16.9 kPa) for 0.6 s and separated with the buffer containing 50 mM ammonium acetate, 0.5% (v/v) acetic acid, and 50% (v/v) acetonitrile in methanol medium at 24 kV applied voltage. The analytes were detected by UV at 214 nm. The two bisbenzylisoquinoline alkaloids can be separated within 6 min and quantified with high sensitivity. The detection limits were 0.30 ng mL(-1) for fangchinoline and 0.34 ng mL(-1) for tetrandrine, which indicated that the sensitivities were at least 1000-fold enhanced over those reported in the literature as obtained by UV detection. The method was applied to the analysis of fangchinoline and tetrandrine in Radix Stephaniae tetrandrae and its medicinal preparations with good results.
建立了一种简单、快速、灵敏的非水毛细管电泳方法，采用柱头场放大进样堆积富集技术分析粉防己碱和防己诺林碱。在甲醇介质中，先以16.9 kPa的压力进样乙醇0.6 s，然后以8 kV进样50 s，再用含50 mM醋酸铵、0.5%（v/v）醋酸和50%（v/v）乙腈的缓冲液在24 kV的施加电压下进行分离，可获得最佳的分离和堆积条件。通过214 nm紫外检测分析物。两种双苄基异喹啉生物碱可在6分钟内分离，并具有高灵敏度定量。粉防己碱的检测限为0.30 ng mL(-1)，防己诺林碱的检测限为0.34 ng mL(-1)，这表明该方法的灵敏度比文献报道的紫外检测法至少提高了1000倍。该方法应用于粉防己及其制剂中粉防己碱和防己诺林碱的分析，结果良好。
