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[盾叶薯蓣同源四倍体生化指标及有效成分分析]

[Analysis of biochemical indices and efficient ingredients in autotetraploid of Dioscorea zingibiernsis].

作者信息

Xie Cai-Xia, Dong Cheng-Ming, Gao Shan-Lin, Qin Hui-Zhen, Bai Yan

机构信息

Henan College of Traditional Chinese Medicine, Zhengzhou 450008, China.

出版信息

Zhong Yao Cai. 2009 Jun;32(6):840-3.

PMID:19764320
Abstract

OBJECTIVE

To improve the yield and quality of Dioscrea zingibiernsis.

METHODS

The Biochemical indices and the diosgenin's content were analysed in the autotetraploid lines.

RESULTS

The results showed that the activities of APX, SOD and POD in most of autotetraploid lines were higher than that in diploid line or close to it, there was also difference between autotetraploid lines and control lines in the SDS-PAGE of soluble proteins. The content of diosgenin in most autotetraploid plantlets were higher than that in the control.

CONCLUSION

There were difference between autotetraploid and control lines in the content of diosgenin and Biochemical indices, therefore, inducing autotetraploid could be an effective way to breeding the superior varieties.

摘要

目的

提高盾叶薯蓣的产量和品质。

方法

对同源四倍体系的生化指标和薯蓣皂苷元含量进行分析。

结果

结果表明,大多数同源四倍体系中APX、SOD和POD的活性高于二倍体系或与之相近,在可溶性蛋白质的SDS-PAGE中同源四倍体系与对照系之间也存在差异。大多数同源四倍体试管苗中薯蓣皂苷元的含量高于对照。

结论

同源四倍体与对照系在薯蓣皂苷元含量和生化指标上存在差异,因此,诱导同源四倍体可能是培育优良品种的有效途径。

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