[Study on purification and property of polysaccharide from Scolopendra subspinipes mutilans].

OBJECTIVE

To obtain polysaccharide from Scolopendra subspinipes mutilans and study its partial properties.

METHODS

A raw polysaccharide was isolated from Chinese medicine Scolopendra subspinipes mutilans, and three groups were isolated by DEAE-52. The thin layer chromatography and gel filtration chromatography were used to detect the main monosaccharide composition and molecular weight of the group I. The inhibitory effect of the group I on tumor cells was detected by MTT assay.

RESULTS

The molecular weight of the group I was 33.1 kD. The inhibitory effect of the group I on Hela cells was obvious, as its inhibitive rate was 60.8% on Hela cells when the polysaccharide's concentration was 3.13 microg/mL, but it had no effect on the Eca 109 cells.

CONCLUSION

The group I has specific effects on different tumor cells.