[少棘蜈蚣的薄层色谱鉴别及抗凝活性定量方法研究]

[Research on TLC identification and anti-coagulant activity quantitatively methods of Scolopendra subspinipes mutilans].

作者信息

Li Tao, Tan Xiao-mei, Long Qun, Chen Fei-long

机构信息

Key Laboratory of Chinese Materia Medica Preparation of Guangdong Province, School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China.

出版信息

Zhong Yao Cai. 2012 May;35(5):686-9.

PMID:23213726

Abstract

OBJECTIVE

To improve the quality standard of Scolopendra subspinipes mutilans by researching the methods of the TLC identification and anti-coagulant activity quantitatively.

METHODS

Identified the free arginine (Arg) and serine (Ser) in scolopendra by TLC, screened the samples preparation process and developed solvent systems; Determined the anti-coagulant activity by method of titration with thrombin and screened the pretreatment methods.

RESULTS

When medicinal materials was extracted by formic acid and 95% ethanol (1:1) with ultrasonic method and developed by n-butanol-acetic acid-water (12:5:4), the spots of Arg and Ser were well separated. Ultrasonic method was suitable for preparation of the anti-coagulant components in Scolopendra subspinipes mutilans and their anti-coagulant activity was determined by method of titration with thrombin could get a well reproducibility, the anti-thrombin activity of testing sample was (14.00 +/- 1.53) U/g and those of three different batch were (13.00 +/- 0.58) U/g, (17.00 +/- 1.15) U/g, (15.67 +/- 1.53) U/g respectively.

CONCLUSION

The methods of TLC identification and anti-coagulant activity quantitatively could be used as a basis for improving the quality standard of Scolopendra subspinipes mutilans.

摘要

目的

通过研究薄层层析（TLC）鉴别方法及定量测定抗凝活性，提高少棘巨蜈蚣的质量标准。

方法

采用TLC法鉴别少棘巨蜈蚣中的游离精氨酸（Arg）和丝氨酸（Ser），筛选样品制备过程及展开剂系统；采用凝血酶滴定法测定抗凝活性并筛选预处理方法。

结果

以甲酸-95%乙醇（1:1）超声提取药材，正丁醇-乙酸-水（12:5:4）展开时，Arg和Ser斑点分离良好。超声法适用于少棘巨蜈蚣抗凝成分的制备，采用凝血酶滴定法测定其抗凝活性重复性良好，供试品抗凝血酶活性为（14.00±1.53）U/g，三个不同批次的分别为（13.00±0.58）U/g、（17.00±1.15）U/g、（15.67±1.53）U/g。