LEC大鼠自发性肝癌发生过程中c-jun基因表达增加。

Increased expression of c-jun gene during spontaneous hepatocarcinogenesis in LEC rats.

作者信息

Suzuki H, Fujita H, Mullauer L, Kuzumaki N, Konaka S, Togashi Y, Takeichi N, Kawamukai Y, Uchino J

机构信息

Laboratory of Molecular Genetics, University School of Medicine, Sapporo, Japan.

出版信息

Cancer Lett. 1990 Sep;53(2-3):205-12. doi: 10.1016/0304-3835(90)90215-j.

DOI:10.1016/0304-3835(90)90215-j

PMID:1976434

Abstract

We have studied the expressions of nine proto-oncogenes (c-myc, N-myc, c-fos, C-jun, p53, H-ras, N-ras, c-raf, hst) and two other genes (PCNA, GST-P) during the spontaneous development of hepatocellular carcinomas (HCCs) in LEC rats. Expression of c-myc, H-ras, N-ras, C-raf, p53 and PCNA genes was detected, but this did not significantly change during the development of HCCs in LEC rats. Expression of N-myc and hst genes was not detectable. Expression of c-fos gene was detected in one HCC case out of four. Significantly increased expression of c-jun gene was observed in the liver tissues of LEC rats aged 8 months. This high expression was decreased with the development of HCCs. On the other hand, the expression of GST-P gene increased in parallel with the clinical course of the development of HCCs in LEC rats. The pattern of c-jun mRNA augmentation was different from that of GST-P mRNA. These observations suggest that c-jun gene may play a role in the spontaneous development of HCCs in LEC rats.

摘要

我们研究了9种原癌基因（c-myc、N-myc、c-fos、C-jun、p53、H-ras、N-ras、c-raf、hst）和另外两种基因（PCNA、GST-P）在LEC大鼠肝细胞癌（HCC）自发发展过程中的表达情况。检测到了c-myc、H-ras、N-ras、C-raf、p53和PCNA基因的表达，但在LEC大鼠HCC发展过程中这并未发生显著变化。未检测到N-myc和hst基因的表达。在4例HCC病例中有1例检测到了c-fos基因的表达。在8月龄LEC大鼠的肝脏组织中观察到C-jun基因表达显著增加。随着HCC的发展，这种高表达降低。另一方面，GST-P基因的表达与LEC大鼠HCC发展的临床进程平行增加。C-jun mRNA增加的模式与GST-P mRNA不同。这些观察结果表明，C-jun基因可能在LEC大鼠HCC的自发发展中起作用。