Todorova R
Institute of Biophysics, Bulgarian Academy of Sciences, 1113 Sofia, Bulgaria.
Prikl Biokhim Mikrobiol. 2009 Jul-Aug;45(4):493-6.
To test the efficiency of some alternative methods for protein delivery into live cells were used rEGFP (recombinant Enhanced Green Fluorescent Protein) and HeLa cells as model system. The compared methods, electroporation (up to 10%) and Bioporter (up to 20%), deliver proteins and peptides to the cells with efficiency, enough for functional studies of mammalian cells. Thus, the delivery by either of both methods, followed by fluorescence activated cell sorting (FACS) of the cell population, can give a sufficient number of uniformly loaded cells for different studies including transcription assays and gene therapy.
为了测试一些将蛋白质递送至活细胞的替代方法的效率，使用了重组增强绿色荧光蛋白（rEGFP）和HeLa细胞作为模型系统。所比较的方法，即电穿孔法（高达10%）和生物转运体法（高达20%），能够有效地将蛋白质和肽递送至细胞，足以用于哺乳动物细胞的功能研究。因此，通过这两种方法中的任何一种进行递送，随后对细胞群体进行荧光激活细胞分选（FACS），可以获得足够数量的均匀负载细胞，用于包括转录分析和基因治疗在内的不同研究。
