Effects of temperature on the immobilization and the initiation of motility of spermatozoa in the male reproductive tract of the domestic fowl, Gallus domesticus.
作者信息
Ashizawa K, Sano R
机构信息
Laboratory of Animal Reproduction, Faculty of Agriculture, Miyazaki University, Japan.
出版信息
Comp Biochem Physiol A Comp Physiol. 1990;96(2):297-301. doi: 10.1016/0300-9629(90)90696-p.
The motility of undiluted fowl spermatozoa taken from testis, epididymis and ductus deferens was negligible at 40 degrees C, around the normal avian body temperature. 2. The immobilization was not permanent and motility was restored by decreasing the temperature to 30 degrees C or by suspending in a NaCl/TES buffer with 2 mM Ca2+, 2 mM HCO3- or 10% seminal plasma at 40 degrees C. 3. Demembranated spermatozoa taken from testis, epididymis and ductus deferens were also immotile at 40 degrees C. However, these spermatozoa were restored the motility at 30 degrees C except testicular spermatozoa. 4. These results suggest that the capacity of movement of fowl spermatozoa can be readily obtained from testis, but that these spermatozoa are immotile due to temperature-dependent immobilization in the male reproductive tract. 5. Furthermore, it is possible that changes in environmental temperature at ejaculation are one of the important exogenous physiological factors of the initiation of fowl sperm motility.
摘要
取自睾丸、附睾和输精管的未稀释家禽精子，在约正常禽体体温40摄氏度时活力可忽略不计。2. 这种固定化并非永久性的，通过将温度降至30摄氏度或在40摄氏度下悬浮于含2 mM Ca2+、2 mM HCO3-或10%精浆的NaCl/TES缓冲液中，活力得以恢复。3. 取自睾丸、附睾和输精管的去膜精子在40摄氏度时也无活力。然而，除睾丸精子外，这些精子在30摄氏度时恢复了活力。4. 这些结果表明，家禽精子的运动能力可轻易从睾丸获得，但这些精子在雄性生殖道中因温度依赖性固定化而无活力。5. 此外，射精时环境温度的变化可能是引发家禽精子活力的重要外源性生理因素之一。
