Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus C, Denmark.
Biomacromolecules. 2009 Oct 12;10(10):2759-66. doi: 10.1021/bm900589r.
We utilize an aqueous extract of fish proteins (FPs) as a coating for minimizing the adsorption of fibrinogen (Fg) and human serum albumin (HSA). The surfaces include stainless steel (SS), gold (Au), silicon dioxide (SiO(2)), and poly(styrene) (PS). The adsorption processes (kinetics and adsorbed mass) are followed by quartz crystal microbalance with dissipation (QCM-D). Complementary surface information is provided by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). QCM-D shows no mass increases to any of the FP-coated surfaces upon treating with Fg or HSA. Also, when Fg- or HSA-coated surfaces are exposed to the FPs, a significant increase in adsorbed mass occurs because the FPs are highly surface-active displacing Fg. Additionally, fluorescence microscopy confirms that very little Fg adsorbs to the FP-coated surfaces. We propose that FP coatings prevent protein adsorption by steric stabilization and could be an alternative method for preventing unwanted bioadhesion on medical materials.
我们利用一种鱼蛋白的水提物（FPs）作为涂层，以最小化纤维蛋白原（Fg）和人血清白蛋白（HSA）的吸附。这些表面包括不锈钢（SS）、金（Au）、二氧化硅（SiO(2)）和聚苯乙烯（PS）。通过石英晶体微天平（QCM-D）来跟踪吸附过程（动力学和吸附质量）。X 射线光电子能谱（XPS）和原子力显微镜（AFM）提供了补充的表面信息。QCM-D 显示，在使用 Fg 或 HSA 处理任何 FP 涂层表面时，没有质量增加。此外，当 Fg 或 HSA 涂层表面暴露于 FPs 时，吸附质量会显著增加，因为 FPs 具有很高的表面活性，可以置换 Fg。此外，荧光显微镜证实，很少有 Fg 吸附到 FP 涂层表面。我们提出 FP 涂层通过空间稳定来防止蛋白质吸附，并且可能是防止医疗材料上不需要的生物黏附的替代方法。
