Department of Neurology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, PR China.
Brain Res. 2009 Dec 11;1302:240-7. doi: 10.1016/j.brainres.2009.09.034. Epub 2009 Sep 16.
Xyloketal B is a novel marine compound with unique chemical structure isolated from mangrove fungus Xylaria sp. (no. 2508). Recently, we have demonstrated that Xyloketal B is an antioxidant and can protect against oxidized low density lipoprotein (LDL)-induced cell injury. In the present study, we investigated whether Xyloketal B can protect against ischemia-induced cell injury in an in vitro oxygen glucose deprivation (OGD) model of ischemic stroke in PC12 cells. We found that Xyloketal B could directly scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical and protect PC12 cells against OGD insult. Furthermore, Xyloketal B alleviated OGD-induced mitochondria superoxide, mitochondria fragmentation and GTPase dynamin-related protein 1 (Drp1) overexpression as well as reduction of mitochondrial membrane potential. All together, the present study demonstrates that Xyloketal B protects PC12 cells against OGD-induced cell injury and that the anti-oxidative property and protective action on mitochondria may account for its neuroprotective actions.
木榴醇 B 是一种从红树林真菌 Xylaria sp.（编号 2508）中分离得到的具有独特化学结构的新型海洋化合物。最近，我们已经证明木榴醇 B 是一种抗氧化剂，可以防止氧化型低密度脂蛋白（LDL）诱导的细胞损伤。在本研究中，我们研究了木榴醇 B 是否可以在 PC12 细胞缺血性中风体外氧葡萄糖剥夺（OGD）模型中保护细胞免受缺血引起的损伤。我们发现木榴醇 B 可以直接清除 1,1-二苯基-2-苦基肼基（DPPH）自由基，并保护 PC12 细胞免受 OGD 损伤。此外，木榴醇 B 减轻了 OGD 诱导的线粒体超氧化物、线粒体片段化和 GTPase 动力相关蛋白 1（Drp1）过表达以及线粒体膜电位降低。综上所述，本研究表明木榴醇 B 可保护 PC12 细胞免受 OGD 诱导的细胞损伤，其抗氧化特性和对线粒体的保护作用可能是其神经保护作用的原因。
