Department of Clinical Pharmacology and Therapeutics, Tohoku University Graduate School of Pharmaceutical Sciences and Medicine, Sendai 980-8578, Japan.
Peptides. 2009 Dec;30(12):2316-22. doi: 10.1016/j.peptides.2009.09.015. Epub 2009 Sep 16.
Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, was associated with organ damage in the kidney. However, there has been little information for (P)RR in hearts. To investigate the regulation of (P)RR in heart failure, we examined the expression of (P)RR in hearts and kidneys of rats with congestive heart failure (CHF) due to coronary ligation by quantitative RT-PCR and immunohistochemistry. Significantly increased levels of (P)RR mRNA were found in the atrium, right ventricle, non-infarcted part of left ventricle, infarcted part of left ventricle and kidney of CHF rats, when compared with sham operated rats (about 1.6-fold, 1.4-fold, 1.6-fold, 1.7-fold and 1.5-fold, respectively). Expression levels of mRNAs encoding renin and angiotensinogen in these heart and kidney tissues were also increased in the CHF rats. Immunohistochemistry showed positive (P)RR immunostaining in the myocardium, the renal tubular cells, and vascular smooth muscle and endothelial cells in the heart and the kidney. The renal tubular cells were more intensely immunostained in CHF rats than in sham operated rats. These findings suggest that the expression of (P)RR is increased in the hearts and kidneys of rats with heart failure, and that (P)RR may contribute to heart failure.
最近的研究表明，（前）肾素受体（(P)RR），肾素-血管紧张素系统的一个新成员，与肾脏的器官损伤有关。然而，（P)RR 在心脏中的信息很少。为了研究（P)RR 在心力衰竭中的调节作用，我们通过定量 RT-PCR 和免疫组织化学方法检查了冠状动脉结扎所致充血性心力衰竭（CHF）大鼠心脏和肾脏中（P)RR 的表达。与假手术大鼠相比，CHF 大鼠的心房、右心室、左心室未梗死部分、左心室梗死部分和肾脏中（P)RR mRNA 的水平显著升高（分别约为 1.6 倍、1.4 倍、1.6 倍、1.7 倍和 1.5 倍）。这些心脏和肾脏组织中编码肾素和血管紧张素原的 mRNAs 的表达水平也在 CHF 大鼠中升高。免疫组织化学显示，（P)RR 在心肌、肾小管细胞以及心脏和肾脏中的血管平滑肌和内皮细胞中呈阳性免疫染色。与假手术大鼠相比，CHF 大鼠的肾小管细胞的免疫染色更强。这些发现表明，心力衰竭大鼠的心脏和肾脏中（P)RR 的表达增加，（P)RR 可能导致心力衰竭。
