由低营养培养基中培养的戴氏不动杆菌 W6 产生和特性化的胞内生物絮凝剂。
Production and characterization of an intracellular bioflocculant by Chryseobacterium daeguense W6 cultured in low nutrition medium.
机构信息
State Key Lab for Agrobiotechnology, Center of Biomass Engineering, Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
出版信息
Bioresour Technol. 2010 Feb;101(3):1044-8. doi: 10.1016/j.biortech.2009.08.108. Epub 2009 Sep 18.
A novel intracellular bioflocculant (named MBF-W6) produced by Chryseobacterium daeguense W6 cultured in low nutrition medium was investigated in this study. The effects of carbon source, nitrogen source, C/N ratio, initial pH, inoculum size, culture temperature and shaking speed on MBF-W6 production were studied. Chemical analysis showed that the purified MBF-W6 was mainly composed of 32.4% protein, 13.1% polysaccharide and 6.8% nucleic acid. Fourier-transform infrared (FTIR) spectroscopy indicated the presence of carboxyl, hydroxyl, and methoxyl groups. The elemental analysis of purified MBF-W6 revealed that the mass proportion of C, H, O, N and S was 40.92:6.53:44.01:8.53:1.01 (w/w) correspondingly. MBF-W6 had good flocculating rate in Kaolin suspension without any cation addition. The highest flocculating rate of 96.9% was achieved under the optimal conditions (bioflocculant dosage 1.2 mg l(-1), pH 5.6 and temperature 15 degrees C).
本研究考察了一种新型胞内生物絮凝剂（命名为 MBF-W6），该生物絮凝剂由在低营养培养基中培养的戴氏不动杆菌 W6 产生。研究了碳源、氮源、C/N 比、初始 pH 值、接种量、培养温度和摇床转速对 MBF-W6 产生的影响。化学分析表明，纯化后的 MBF-W6 主要由 32.4%的蛋白质、13.1%的多糖和 6.8%的核酸组成。傅里叶变换红外（FTIR）光谱表明存在羧基、羟基和甲氧基。纯化的 MBF-W6 的元素分析表明，C、H、O、N 和 S 的质量比例分别为 40.92:6.53:44.01:8.53:1.01（w/w）。MBF-W6 在没有任何阳离子添加的情况下对高岭土悬浮液具有良好的絮凝率。在最佳条件下（生物絮凝剂用量为 1.2mg l(-1)、pH 值为 5.6 和温度为 15 摄氏度），絮凝率最高可达 96.9%。
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