Department of Pharmacy and Laboratory, Huizhou Health Sciences Polytechnic, Huizhou, China.
College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.
Pol J Microbiol. 2022 Sep 24;71(3):341-351. doi: 10.33073/pjm-2022-030. eCollection 2022 Sep 1.
A preliminary study was carried out to optimize the culture medium conditions for producing a novel microbial flocculant from the marine bacterial species . The optimal glucose, yeast extract, and glutamate contents were 30, 10, and 2 g/l, respectively, while the optimal initial pH of the culture medium was determined to be 8. Following response surface optimization, the maximum bioflocculant production level of 1.36 g/l was achieved, which was 43.40% higher than the original culture medium. Within 5 min, a 20.0% (v/v) dosage of the yielded bioflocculant applied to algal cultures resulted in the highest flocculating efficiency of 93.9% with . The bioflocculant from MCCC1113 may have promising application potential for highly productive microalgae collection, according to the findings of this study.
本研究旨在优化海洋细菌 MCCC1113 产生新型微生物絮凝剂的发酵培养基条件。实验确定最佳葡萄糖、酵母膏和谷氨酸浓度分别为 30、10 和 2 g/L，最佳初始培养基 pH 值为 8。通过响应面优化，生物絮凝剂产量达到 1.36 g/L，比原始培养基提高了 43.40%。在 5 min 内，向藻类培养液中投加 20.0%（v/v）剂量的该生物絮凝剂，其絮凝效率最高可达 93.9%。本研究表明，MCCC1113 产生的生物絮凝剂可能具有在高效微藻收集方面有很好的应用潜力。
