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一株新型生物絮凝剂产生菌 MCCC1113 的发酵条件优化及其在微藻收获中的絮凝性能评价

A Novel Bioflocculant Produced by MCCC1113: Optimization of Fermentation Conditions by Response Surface Methodology and Evaluation of Flocculation Performance when Harvesting Microalgae.

机构信息

Department of Pharmacy and Laboratory, Huizhou Health Sciences Polytechnic, Huizhou, China.

College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

出版信息

Pol J Microbiol. 2022 Sep 24;71(3):341-351. doi: 10.33073/pjm-2022-030. eCollection 2022 Sep 1.

Abstract

A preliminary study was carried out to optimize the culture medium conditions for producing a novel microbial flocculant from the marine bacterial species . The optimal glucose, yeast extract, and glutamate contents were 30, 10, and 2 g/l, respectively, while the optimal initial pH of the culture medium was determined to be 8. Following response surface optimization, the maximum bioflocculant production level of 1.36 g/l was achieved, which was 43.40% higher than the original culture medium. Within 5 min, a 20.0% (v/v) dosage of the yielded bioflocculant applied to algal cultures resulted in the highest flocculating efficiency of 93.9% with . The bioflocculant from MCCC1113 may have promising application potential for highly productive microalgae collection, according to the findings of this study.

摘要

本研究旨在优化海洋细菌 MCCC1113 产生新型微生物絮凝剂的发酵培养基条件。实验确定最佳葡萄糖、酵母膏和谷氨酸浓度分别为 30、10 和 2 g/L,最佳初始培养基 pH 值为 8。通过响应面优化,生物絮凝剂产量达到 1.36 g/L,比原始培养基提高了 43.40%。在 5 min 内,向藻类培养液中投加 20.0%(v/v)剂量的该生物絮凝剂,其絮凝效率最高可达 93.9%。本研究表明,MCCC1113 产生的生物絮凝剂可能具有在高效微藻收集方面有很好的应用潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/790c/9608167/a988297cc015/pjm-71-341-g001a.jpg

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