Luo Zhengshan, Chen Li, Chen Changhong, Zhang Wei, Liu Ming, Han Ye, Zhou Jiangang
School of Environmental Engineering, Wuhan Textile University, FangZhi Road, Wuhan, 430073, China.
Appl Biochem Biotechnol. 2014 Feb;172(3):1282-92. doi: 10.1007/s12010-013-0601-8. Epub 2013 Oct 30.
The production and characterization of a bioflocculant, MBF-6, by Klebsiella pneumoniae was investigated. Optimum culture conditions for bioflocculant production were an initial medium pH of 7, an incubation temperature of 30 °C, and an inoculum size of 1% (v/v) of cell density 1.0 × 10(8) cfu/mL. The carbon, nitrogen, and cation sources for optimum bioflocculant production were glucose, peptone, and ZnCl₂. The bioflocculant mainly consisted of protein (3.4%) and sugar (95.1%). Fourier transform infrared (FTIR) spectrum revealed the presence of carboxyl and hydroxyl groups while the thermogravimetric analysis (TGA) showed a degradation temperature (T(d)) of 81.4 °C. MBF-6 had a good flocculating rate in kaolin suspension without cation addition and was stable over a wide range of pH and temperature. Investigation on the flocculation efficacy of the characterized MBF-6 for wastewater treatment of dairy, woolen, brewery, and sugar industries suggested it to be effective.
研究了肺炎克雷伯菌产生生物絮凝剂MBF - 6及其特性。生物絮凝剂生产的最佳培养条件为：初始培养基pH值为7，培养温度为30℃，接种量为细胞密度1.0×10⁸ cfu/mL的1%（v/v）。最佳生物絮凝剂生产的碳源、氮源和阳离子源分别为葡萄糖、蛋白胨和ZnCl₂。该生物絮凝剂主要由蛋白质（3.4%）和糖（95.1%）组成。傅里叶变换红外光谱（FTIR）显示存在羧基和羟基，而热重分析（TGA）显示降解温度（T(d)）为81.4℃。MBF - 6在不添加阳离子的高岭土悬浮液中具有良好的絮凝率，并且在较宽pH和温度范围内稳定。对经表征的MBF - 6用于乳制品、羊毛、啤酒厂和制糖工业废水处理的絮凝效果研究表明其具有有效性。
