Kawabe Y, Ochi A
Department of Immunology and Medical Genetics, University of Toronto, Ontario, Canada.
J Exp Med. 1990 Oct 1;172(4):1065-70. doi: 10.1084/jem.172.4.1065.
The cellular basis of the in vitro and in vivo T cell responses to Staphylococcus enterotoxin B (SEB) has been investigated. The proliferation and cytotoxicity of V beta 8.1,2+,CD4+ and CD8+ T cells were observed in in vitro response to SEB. In primary cytotoxicity assays, CD4+ T cells from control spleens were more active than their CD8+ counterparts, however, in cells derived from SEB-primed mice, CD8+ T cells were dominant in SEB-specific cytotoxicity. In vivo priming with SEB abrogated the response of V beta 8.1,2+,CD4+ T cells despite the fact that these cells exist in significant number. This SEB-specific anergy occurred only in V beta 8.1,2+,CD4+ T cells but not in CD8+ T cells. These findings indicate that the requirement for the induction of antigen-specific anergy is different between CD4+ and CD8+ T cells in post-thymic tolerance, and the existence of coanergic signals for the induction of T cell anergy is suggested.
已对体外和体内T细胞对葡萄球菌肠毒素B(SEB)反应的细胞基础进行了研究。在体外对SEB的反应中,观察到Vβ8.1,2 +、CD4 +和CD8 + T细胞的增殖和细胞毒性。在初次细胞毒性试验中,来自对照脾脏的CD4 + T细胞比其CD8 +对应细胞更活跃,然而,在源自经SEB致敏小鼠的细胞中,CD8 + T细胞在SEB特异性细胞毒性中占主导地位。尽管存在大量Vβ8.1,2 +、CD4 + T细胞,但用SEB进行体内致敏可消除这些细胞的反应。这种SEB特异性无反应性仅发生在Vβ8.1,2 +、CD4 + T细胞中,而不发生在CD8 + T细胞中。这些发现表明,在胸腺后耐受性中,CD4 +和CD8 + T细胞诱导抗原特异性无反应性的要求不同,提示存在诱导T细胞无反应性的共无反应性信号。