de Araujo José M, Marques Edmilson J, de Oliveira José V
Depto. de Agronomia, Univ. Federal Rural de Pernambuco, Dos Irmaos, Recife, PE.
Neotrop Entomol. 2009 Jul-Aug;38(4):520-5. doi: 10.1590/s1519-566x2009000400014.
This work aimed to determine the efficiency of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana to control the aphid Lipaphis erysimi (Kalt.) (Hemiptera: Aphididae) in kale Brassica oleracea var acephala D.C., as well as their compatibility with a neem oil formulation (Neemseto). Ten isolates of both fungi were tested and the most pathogenic ones were B. bassiana CG001 and M. anisopliae CG30 with 90% and 4.4 days, and 64% and 3.8 days of mortality and median lethal time, respectively. Bioassays with neem at concentrations of 0.5, 1.0 and 2.0% were done either by leaf discs dipping or spraying the aphids on the leaf discs. The neem spraying treatment at 2.0% provided 90% mortality. The use of B. bassiana isolate CG001 or M. anisopliae isolate CG30 with neem at 0.125, 0.25, and 0.5%, demonstrated that these isolates could have their spore viability or colony growth affected when exposed to neem concentrations higher than 0.25%. In absolute values, the isolates B. bassiana CG001 and M. anisopliae CG30 are the most virulent to L. erysimi, and could be utilized in the management of this pest.
本研究旨在确定昆虫病原真菌绿僵菌和白僵菌对羽衣甘蓝上的蚜虫甘蓝蚜（半翅目：蚜科）的防治效果，以及它们与印楝油制剂（Neemseto）的兼容性。对这两种真菌的十个分离株进行了测试，其中致病性最强的是白僵菌CG001和绿僵菌CG30，其死亡率分别为90%和64%，中位致死时间分别为4.4天和3.8天。采用叶碟浸渍法或在叶碟上喷洒蚜虫的方式，对浓度为0.5%、1.0%和2.0%的印楝进行了生物测定。2.0%的印楝喷洒处理死亡率达90%。使用白僵菌分离株CG001或绿僵菌分离株CG30与浓度为0.125%、0.25%和0.5%的印楝混合，结果表明，当暴露于高于0.25%的印楝浓度时，这些分离株的孢子活力或菌落生长会受到影响。绝对值上，白僵菌CG001和绿僵菌CG30分离株对甘蓝蚜的毒性最强，可用于该害虫的治理。
