Crop Protection Unit, Department of Agriculture and Food Science, Science and Technology Centre, University of La Rioja, C/Madre de Dios, 51, 26006 Logroño, Spain.
Exp Appl Acarol. 2012 Dec;58(4):395-405. doi: 10.1007/s10493-012-9594-1. Epub 2012 Jun 29.
Laboratory studies were developed to evaluate the compatibility of flufenoxuron and azadirachtin with Beauveria bassiana against Tetranychus urticae larvae along with the required Probit analysis of the involved chemicals on all of the life stages of this mite. Flufenoxuron displayed parallel regression lines for the mortality of eggs, deutonymphs and adults. Larvae and protonymphs were the most susceptible life stages. Protonymphs were 35 times more sensitive than eggs and adults. Azadirachtin gave equal mortality on proto- and deutonymphs. The response of eggs and adults was equivalent when treated with azadirachtin. The regression lines for proto- and deutonymphs were parallel to those of adults and eggs yet three times more sensitive. The effects of separate combinations of the entomopathogenic fungus Beauveria bassiana at its LC(20) with flufenoxuron and azadirachtin at their corresponding LC(40) were evaluated on mite larvae. The application of flufenoxuron with B. bassiana revealed a clear synergy. While the combination of azadirachtin and B. bassiana had an additive effect. These combinations with B. bassiana could improve mite control by contributing to a decline in the likelihood of resistance so often described in the literature.
实验室研究旨在评估 flufenoxuron 和印楝素与 Beauveria bassiana 对 Tetranychus urticae 幼虫的相容性，以及对该螨所有生命阶段的相关化学物质进行所需的 Probit 分析。flufenoxuron 对卵、若虫和成虫的死亡率呈平行回归线。幼虫和前若虫是最敏感的生命阶段。前若虫比卵和成虫敏感 35 倍。印楝素对原若虫和若虫的致死率相等。用印楝素处理时，卵和成虫的反应相同。原若虫和若虫的回归线与成虫和卵的回归线平行，但敏感三倍。分别用昆虫病原真菌 Beauveria bassiana 在其 LC(20)与 flufenoxuron 和 azadirachtin 在其相应的 LC(40)组合处理螨幼虫，评估其效果。flufenoxuron 与 B. bassiana 的联合应用显示出明显的增效作用。而印楝素与 B. bassiana 的组合则具有相加作用。这些与 B. bassiana 的组合可以通过降低抗性的可能性来改善螨类的控制，这在文献中经常被描述。
