Sloboda Roger D
Department of Biological Sciences, Dartmouth College, Hanover, NH, USA.
Methods Mol Biol. 2009;586:207-25. doi: 10.1007/978-1-60761-376-3_11.
The growth and maintenance of almost all cilia and flagella are dependent on the proper functioning of the process of intraflagellar transport (IFT). This includes the primary cilia of most cells in humans that are in interphase or the G(o) phase of the cell cycle. The model system for the study of IFT is the flagella of the bi-flagellate green alga Chlamydomonas. It is in this organism that IFT was first discovered, and genetic data from a Chlamydomonas mutant first linked the process of IFT to polycystic kidney disease in humans. The information given in this chapter addresses procedures to purify IFT particles from flagella and localize these particles, and their associated motor proteins, in flagella using light and electron microscopic approaches.
几乎所有纤毛和鞭毛的生长与维持都依赖于鞭毛内运输（IFT）过程的正常运作。这包括处于细胞周期间期或G（0）期的人类大多数细胞的初级纤毛。用于研究IFT的模型系统是双鞭毛绿藻衣藻的鞭毛。正是在这种生物体中首次发现了IFT，并且来自衣藻突变体的遗传数据首次将IFT过程与人类多囊肾病联系起来。本章提供的信息涉及从鞭毛中纯化IFT颗粒的程序，以及使用光学和电子显微镜方法在鞭毛中定位这些颗粒及其相关运动蛋白的方法。
