Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
Breast Cancer Res Treat. 2010 May;121(1):241-4. doi: 10.1007/s10549-009-0548-1. Epub 2009 Sep 19.
A lasting legacy of a fetus to the mother is a small number of stably persistent allogeneic cells; the phenomenon known as fetal microchimerism. Prior studies demonstrated that fetal microchimerism in the peripheral blood is associated with protection from breast cancer. Whether the same association of fetal microchimerism extends to the tissue of interest, the breast, is unknown. Total genomic DNA was extracted from frozen normal breast tissue adjacent to invasive disease in women with breast cancer. Control DNA was extracted from reduction mammoplasty tissues from women with no prior history of any breast cancer. The presence of male DNA, presumably from a prior male fetus, was determined with a quantitative PCR assay for the Y chromosome gene, DYS14. Proportions of tissues harboring fetal microchimerism were compared. Thirty-eight cancer-free breast tissues from women with and without a history of breast cancer were evaluable for the presence and quantity of fetal microchimerism testing with the DYS14 assay. Breast tissue from women free of cancer harbored FMc more frequently than normal breast tissue adjacent to invasive disease in women with breast cancer (63 and 26%, respectively). The odds ratio, corrected for total DNA quantity tested, for this protective association of fetal microchimerism against breast cancer was 0.17 (95% confidence interval 0.04-0.76). Findings indicate that the protective association of fetal microchimerism against breast cancer observed previously in the peripheral blood is also reflected in breast tissue.
胎儿向母亲传递的持久遗产是少量稳定持久的同种异体细胞；这种现象被称为胎儿微嵌合体。先前的研究表明，外周血中的胎儿微嵌合体与乳腺癌的保护有关。同样的胎儿微嵌合体与感兴趣的组织（乳房）之间的关联是否存在尚不清楚。从患有乳腺癌的女性浸润性疾病附近的冷冻正常乳房组织中提取总基因组 DNA。从没有任何乳腺癌病史的女性乳房缩小手术组织中提取对照 DNA。使用定量 PCR 测定 Y 染色体基因 DYS14 确定是否存在来自先前男性胎儿的男性 DNA。比较了携带胎儿微嵌合体的组织比例。用 DYS14 测定法对 38 例无癌症的乳腺癌组织进行了评估，这些组织来自有或没有乳腺癌病史的女性，以检测胎儿微嵌合体的存在和数量。无癌症的女性乳房组织比患有乳腺癌的女性浸润性疾病附近的正常乳房组织更常含有 FMc（分别为 63%和 26%）。经测试总 DNA 量校正后，胎儿微嵌合体对乳腺癌的这种保护关联的比值比为 0.17（95%置信区间为 0.04-0.76）。研究结果表明，先前在外周血中观察到的胎儿微嵌合体对乳腺癌的保护关联也反映在乳房组织中。
