Kim Youngmi, Hendrickson Rick, Mosier Nathan S, Ladisch Michael R
Department of Agricultural and Biological Engineering, and Laboratory of Renewable Resources Engineering, Purdue University, West Lafayette, IN, USA.
Methods Mol Biol. 2009;581:93-102. doi: 10.1007/978-1-60761-214-8_7.
Lignocellulosic biomass is an abundant and renewable resource for fuel ethanol production. However, the lignocellulose is recalcitrant to enzymatic hydrolysis because of its structural complexity. Controlled-pH liquid hot water (LHW) pretreatment of cellulosic feedstock improves its enzymatic digestibility by removing hemicellulose and making the cellulose more accessible to cellulase enzymes. The removed hemicellulose is solubilized in the liquid phase of the pretreated feedstock as oligosaccharides. Formation of monomeric sugars during the LHW pretreatment is minimal. The LHW pretreatment is carried out by cooking the feedstock in process water at temperatures between 160 and 190 degrees C and at a pH of 4-7. No additional chemicals are needed. This chapter presents the detailed procedure of the LHW pretreatment of lignocellulosic biomass.
木质纤维素生物质是用于生产燃料乙醇的丰富且可再生的资源。然而，由于其结构复杂性，木质纤维素对酶水解具有抗性。对纤维素原料进行可控pH的液态热水（LHW）预处理，通过去除半纤维素并使纤维素更易被纤维素酶作用，从而提高其酶消化率。去除的半纤维素以寡糖的形式溶解在预处理原料的液相中。LHW预处理过程中单体糖的形成极少。LHW预处理是通过在工艺用水中于160至190摄氏度的温度和4至7的pH下蒸煮原料来进行的。无需额外添加化学物质。本章介绍了木质纤维素生物质LHW预处理的详细步骤。
