Yuan Wei-li, Qin Xing-jun, Wang Xu-kai
Dept. of Stomatology, The Fourth Affiliated Hospital, China Medical University, Shenyang 110032, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Aug;27(4):361-5.
To investigate the expression and correlation of mast cell, Clusterin/apoJ and transforming growth factor-beta (TGF-beta) in the different stages of human dermal hemangioma.
Immunohistochemical stain technique (SABC) and toluidine blue (TB) stain technique were respectively used to detect the expression level of Clusterin/apoJ and TGF-beta and the number of mast cells in the different stages of human dermal hemangioma.
There was remarkable statistical difference between the advanced stage of proliferative hemangioma and the other stages of proliferative hemangioma in the number of mast cell(P<0.01). There was also remarkable statistical difference between the early stage of involutional hemangioma and the other stages of involutional hemangioma in the number of mast cell (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the advanced stage of proliferative hemangioma was significantly higher than the other stages in proliferative hemangioma (P<0.01). The expression of Clusterin/apoJ and TGF-beta in the early stage of involutional hemangioma was significantly higher than the other stages in involutional hemangioma (P<0.01). There was a significantly positive correlation between Clusterin/apoJ and TGF-beta in the different stages of human dermal hemangioma (P<0.01). The expression level of Clusterin/apoJ and TGF-beta was positively correlated with the number of mast cell in the different stages of human dermal hemangioma (P<0.01).
Mast cell may play a promotive role of apoptosis during the spontaneous regulate the expression of Clusterin/apoJ and promote the spontaneous involution of human dermal hemangioma.
探讨肥大细胞、凝聚素/载脂蛋白J（Clusterin/apoJ）和转化生长因子-β（TGF-β）在人类皮肤血管瘤不同阶段的表达及相关性。
分别采用免疫组织化学染色技术（SABC）和甲苯胺蓝（TB）染色技术检测人类皮肤血管瘤不同阶段Clusterin/apoJ和TGF-β的表达水平以及肥大细胞数量。
增殖期血管瘤晚期与增殖期血管瘤其他阶段相比，肥大细胞数量有显著统计学差异（P<0.01）。消退期血管瘤早期与消退期血管瘤其他阶段相比，肥大细胞数量也有显著统计学差异（P<0.01）。增殖期血管瘤晚期Clusterin/apoJ和TGF-β的表达显著高于增殖期血管瘤其他阶段（P<0.01）。消退期血管瘤早期Clusterin/apoJ和TGF-β的表达显著高于消退期血管瘤其他阶段（P<0.01）。人类皮肤血管瘤不同阶段Clusterin/apoJ与TGF-β之间存在显著正相关（P<0.01）。人类皮肤血管瘤不同阶段Clusterin/apoJ和TGF-β的表达水平与肥大细胞数量呈正相关（P<0.01）。
肥大细胞在人类皮肤血管瘤自发消退过程中可能对凋亡起促进作用，调节Clusterin/apoJ的表达，促进人类皮肤血管瘤的自发消退。
