Tang Tian, Zhang Duan-Lian
Department of Oncology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Oncol Lett. 2017 May;13(5):2937-2944. doi: 10.3892/ol.2017.5856. Epub 2017 Mar 13.
Hemangioma is one of the most common types of infantile vascular benign tumor. The aim of the present study was to investigate the role of B-cell lymphoma 2 (Bcl-2) and tumor protein p53 (p53) in the proliferation and apoptosis of hemangioma cells. A total of 38 paraffin-embedded hemangioma specimens (16 males and 22 females) and another 5 paraffin-embedded healthy surrounding tissue samples, collected between January 2007 and December 2010, were obtained from the Department of Pathology at Renmin Hospital of Wuhan University (Wuhan, China). Immunohistochemistry, hematoxylin and eosin staining, and quantum dot double staining were used to detect the expression of proliferating cell nuclear antigen (PCNA), Bcl-2 and p53 in hemangioma and healthy surrounding skin tissue samples. All hemangioma specimens were classified into proliferative or the involuting stage hemangioma according to Mulliken's criteria and their expression of PCNA. The results of the quantum dot double staining were analyzed using a multi-spectral imaging system. One-way analysis of the variance and the Student-Newman-Keuls q test were performed to statistically analyze the data. There were 24 cases of proliferative stage and 14 cases of involuting stage hemangioma among the specimens. Immunohistochemical analysis results indicated a high expression of Bcl-2 and p53 in proliferative stage hemangioma tissue samples, and low expression in involuting stage hemangioma and healthy tissue samples. Statistical analysis of the results from quantum dot double staining demonstrated that the expression of Bcl-2 and p53 in proliferative hemangioma was significantly increased compared with that in involuting stage specimens (P<0.05) and healthy tissue samples (P<0.05). No significant difference in Bcl-2 and p53 expression was identified between the involuting hemangioma and healthy surrounding tissue samples. The higher expression of Bcl-2 and p53 in proliferative hemangioma suggests that Bcl-2 may cause an imbalance between endothelial cell proliferation and apoptosis through the inhibition of endothelial cell apoptosis. Furthermore, p53 may promote the proliferation of endothelial cells in proliferative hemangioma.
血管瘤是婴幼儿血管性良性肿瘤中最常见的类型之一。本研究的目的是探讨B细胞淋巴瘤2（Bcl-2）和肿瘤蛋白p53（p53）在血管瘤细胞增殖和凋亡中的作用。2007年1月至2010年12月期间，从武汉大学人民医院（中国武汉）病理科获取了38例石蜡包埋的血管瘤标本（男16例，女22例）以及另外5例石蜡包埋的健康周围组织样本。采用免疫组织化学、苏木精-伊红染色和量子点双重染色法检测血管瘤及健康周围皮肤组织样本中增殖细胞核抗原（PCNA）、Bcl-2和p53的表达。根据Mulliken标准及其PCNA表达情况，将所有血管瘤标本分为增殖期或消退期血管瘤。使用多光谱成像系统分析量子点双重染色结果。进行单因素方差分析和Student-Newman-Keuls q检验对数据进行统计学分析。标本中增殖期血管瘤24例，消退期血管瘤14例。免疫组织化学分析结果显示，Bcl-2和p53在增殖期血管瘤组织样本中高表达，在消退期血管瘤和健康组织样本中低表达。量子点双重染色结果的统计学分析表明，增殖期血管瘤中Bcl-2和p53的表达与消退期标本（P<0.05）和健康组织样本（P<0.05）相比显著增加。消退期血管瘤与健康周围组织样本之间Bcl-2和p53表达无显著差异。增殖期血管瘤中Bcl-2和p53的高表达表明，Bcl-2可能通过抑制内皮细胞凋亡导致内皮细胞增殖与凋亡失衡。此外，p53可能促进增殖期血管瘤中内皮细胞的增殖。
