Supabphol Athikom, Muangman Verasing, Chavasiri Warinthorn, Supabphol Roongtawan, Gritsanapan Wandee
Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
J Med Assoc Thai. 2009 Sep;92(9):1171-7.
Bladder cancer is not only a major public health and economically burden for the patients but also a major clinical impact for Thai urologists. The authors' aim was to study the anti-metastatic effect of N-acetylcysteine (NAC), one of the cheap, safe and widely used over-the-counter-drugs in Thailand, on the human bladder cancer cells.
Effects of NAC at various concentrations on the growth, adhesion, migration, and invasion of the human bladder cancer cell line were assessed in vitro.
NAC at the concentrations of 5, 10, 20 and 30 mM could directly and significantly inhibit the growth, adhesion, migration, and invasion of the human bladder cancer cells in a dose-dependent manner The 50% inhibitory concentration (IC50) value for cell viability was 33.33 +/- 0.78 mM. The inhibitory effects on migration, invasion and adhesion properties of the cancer cells were dramatically observed at the concentrations of > or = 10, > or = 20 and > or = 30 mM respectively.
NAC has an anti-metastatic effect on the human bladder cancer cells by inhibiting their growth, adhesion, migration, and invasion properties. This implies the high possibility that the urologists may apply the results to use it intravesically before, during and after the transurethral resection of bladder tumour in addition to its conventional usage by oral and parenteral routes.
膀胱癌不仅给患者带来重大的公共卫生和经济负担，对泰国泌尿外科医生而言也是一项重大的临床挑战。作者旨在研究N-乙酰半胱氨酸（NAC）对人膀胱癌细胞的抗转移作用，NAC是泰国一种廉价、安全且广泛使用的非处方药物。
在体外评估不同浓度的NAC对人膀胱癌细胞系生长、黏附、迁移和侵袭的影响。
5、10、20和30 mM浓度的NAC能够直接且显著地抑制人膀胱癌细胞的生长、黏附、迁移和侵袭，呈剂量依赖性。细胞活力的50%抑制浓度（IC50）值为33.33 +/- 0.78 mM。分别在≥10 mM、≥20 mM和≥30 mM浓度下，对癌细胞迁移、侵袭和黏附特性的抑制作用显著。
NAC通过抑制人膀胱癌细胞的生长、黏附、迁移和侵袭特性，对其具有抗转移作用。这意味着泌尿外科医生除了可将其按常规口服和肠胃外途径使用外，还有很大可能性在经尿道膀胱肿瘤切除术前、术中和术后将其用于膀胱内灌注。
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