以及大剂量维生素C对小鼠肿瘤的评估。
and assessment of high-dose vitamin C against murine tumors.
作者信息
Wang Guoping, Yin Tao, Wang Yongsheng
机构信息
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, Sichuan 610041, P.R. China.
出版信息
Exp Ther Med. 2016 Nov;12(5):3058-3062. doi: 10.3892/etm.2016.3707. Epub 2016 Sep 16.
Vitamin C is widely used in clinical settings and is well known for its safety. Previous studies have shown the efficacy of intravenous vitamin C; however, intratumoral delivery of vitamin C has yet to be attempted. In the present study, the biological effects of high-dose vitamin C on tumor cells were investigated by using the MTT assay and flow cytometry. When administered , high-dose vitamin C inhibited the proliferation of murine colon and breast cancer cells, and induced tumor cell apoptosis. Cytotoxicity of vitamin C was partially reversed by N-acetyl-cysteine at a relatively low dosage. In addition, synergistic anti-tumor effects of vitamin C and cisplatin were observed. In vivo, intratumoral delivery of vitamin C delayed tumor growth in murine solid tumor models. Considering its low toxicity and availability, the present study indicates that vitamin C may be a novel therapeutic method for patients with advanced tumors.
维生素C在临床环境中被广泛使用，并且因其安全性而广为人知。先前的研究已经表明静脉注射维生素C的疗效；然而，肿瘤内递送维生素C尚未有人尝试过。在本研究中，通过使用MTT法和流式细胞术研究了高剂量维生素C对肿瘤细胞的生物学效应。当给予高剂量维生素C时，它抑制了小鼠结肠癌细胞和乳腺癌细胞的增殖，并诱导肿瘤细胞凋亡。维生素C的细胞毒性在相对较低剂量下被N-乙酰半胱氨酸部分逆转。此外，还观察到维生素C和顺铂的协同抗肿瘤作用。在体内，肿瘤内递送维生素C延缓了小鼠实体瘤模型中的肿瘤生长。考虑到其低毒性和可得性，本研究表明维生素C可能是晚期肿瘤患者的一种新型治疗方法。
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