Department of Chemical and Biological Engineering, Missouri University of Science and Technology, 143 Schrenk Hall, Rolla, Missouri 65409, USA.
Langmuir. 2009 Oct 20;25(20):12308-14. doi: 10.1021/la9015349.
One of the most interesting applications for carbon nanotubes is as a support material for bioanalytical devices. In this work, we successfully used an ultraviolet light initiated "graft from" polymerization method to fabricate polymer functionalized carbon nanotubes (PFCNTs) with pendant chains of various functionalities, including poly(ethylene glycol) chains to boost dispersibility and pendant epoxy groups for protein conjugate sites. A model enzyme, alkaline phosphatase, was used to study biomolecule loading efficiency as well as the retention of enzyme activity. Samples with various ratios of the two monomers were fabricated to optimize their use in aqueous environments, and an optimal composition was determined. This method allows the enhancement of enzyme loading amount while retaining high enzyme activity. The morphology of the carbon nanotubes were characterized by STEM and AFM before and after functionalization. In addition, the resulting PFCNTs were analyzed by FT-IR, TGA, and XPS.
碳纳米管最有趣的应用之一是作为生物分析器件的支撑材料。在这项工作中，我们成功地使用紫外光引发的“从接枝”聚合方法制备了具有各种功能侧链的聚合物功能化碳纳米管（PFCNTs），包括聚乙二醇链以提高分散性和用于蛋白质偶联位点的环氧侧链。使用模型酶碱性磷酸酶研究生物分子的负载效率以及酶活性的保留情况。制备了具有不同两种单体比例的样品以优化其在水相环境中的应用，并确定了最佳组成。该方法允许在保持高酶活性的同时提高酶的负载量。在功能化前后，通过 STEM 和 AFM 对碳纳米管的形态进行了表征。此外，还通过 FT-IR、TGA 和 XPS 对所得 PFCNTs 进行了分析。
