比较微切割的霍奇金/里斯滕伯格细胞和霍奇金细胞系与 CD77+B 细胞的 miRNA 图谱，揭示了一组不同表达 miRNA 的独特子集。

Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs.

机构信息

Centre for Medical Genetics (CMGG), Ghent University Hospital, Ghent, Belgium.

出版信息

Br J Haematol. 2009 Dec;147(5):686-90. doi: 10.1111/j.1365-2141.2009.07909.x. Epub 2009 Sep 22.

DOI:10.1111/j.1365-2141.2009.07909.x

PMID:19775296

Abstract

Classical Hodgkin lymphoma (cHL) is characterized by the presence of malignant Hodgkin and Reed Sternberg (HRS) cells. The scarcity of tumour cells in lymphoma biopsies has hampered genetic analyses of HRS cells, including microRNA (miRNA) expression profiling. We determined the expression of 360 miRNAs in microdissected HRS cells from nine cHL patients. These miRNA profiles were compared to those from four cHL cell lines and CD77+ B-cells, yielding a distinct cHL signature of 12 over- and three underexpressed miRNAs. Our data suggest that miRNAs are implicated in the pathogenesis of Hodgkin lymphoma and prompt further investigations concerning their role in cHL.

摘要

经典型霍奇金淋巴瘤（cHL）的特征是存在恶性霍奇金和里德-斯特恩伯格（HRS）细胞。淋巴瘤活检中肿瘤细胞的稀少阻碍了 HRS 细胞的遗传分析，包括 microRNA（miRNA）表达谱分析。我们在 9 例 cHL 患者的微切割 HRS 细胞中测定了 360 个 miRNA 的表达。将这些 miRNA 图谱与 4 个 cHL 细胞系和 CD77+B 细胞进行比较，得出 12 个上调和 3 个下调的 miRNA 的独特 cHL 特征。我们的数据表明，miRNAs 参与了霍奇金淋巴瘤的发病机制，并促使进一步研究它们在 cHL 中的作用。