Department of Endocrinology and Tissue Culture, Institute of Zoology, Jagiellonian University, Kraków, Poland.
Acta Histochem. 2010 Nov;112(6):576-82. doi: 10.1016/j.acthis.2009.06.003. Epub 2009 Sep 22.
Immunolocalization of 3β-hydroxysteroid dehydrogense (3β-HSD), cytochrome P450c17 (P450c17) and androgen receptor (AR) were investigated in rat cumuli oophori (COCs) of late pre-ovulatory follicles and in post-ovulatory COCs bearing fertilized oocytes. A gradient of intensity of 3β-HSD immunolabelling was observed in the granulosa layer of pre-ovulatory follicles, with almost negative immunolabelling in COCs and with the strongest immunoreaction in the mural granulosa cells. Post-ovulatory COCs showed strong 3β-HSD immunolabelling in the peripheral regions and weak labelling near the oocyte, suggestive of responsiveness of cumulus cells to an anti-luteinizing effect exerted by the fertilized oocyte. In pre-ovulatory follicles, a weak P450c17 immunopositivity was limited to expanded cumulus granulosa cells and the positive labelling persisted in post-ovulatory COCs. P450c17 immunopositivity was also found in ampullary epithelial cells. A strong AR immunopositivity was confined mainly to the COCs in pre-ovulatory follicles and a similar immunoreaction was present in the granulosa cells of ovulated COCs. Simultaneous AR and cytochrome P450c17 immunolabelling in the pre- and post-ovulatory COCs is suggestive of an intra- and paracrine androgen regulation of the cumulus granulosa cell function.
3β-羟甾脱氢酶（3β-HSD）、细胞色素 P450c17（P450c17）和雄激素受体（AR）在大鼠晚前期排卵卵泡的卵丘细胞（COCs）和带有受精卵的排卵后 COCs 中的免疫定位进行了研究。在前期排卵卵泡的颗粒层中观察到 3β-HSD 免疫标记的强度梯度，COCs 中几乎没有免疫标记，而壁颗粒细胞中免疫反应最强。排卵后 COCs 在周边区域显示出强烈的 3β-HSD 免疫标记，而在卵母细胞附近标记较弱，提示卵丘细胞对受精卵发挥的抗黄体化作用有反应性。在前期排卵卵泡中，较弱的 P450c17 免疫阳性仅限于扩展的卵丘颗粒细胞，并且这种阳性标记在排卵后 COCs 中持续存在。P450c17 免疫阳性也在壶腹上皮细胞中发现。强烈的 AR 免疫阳性主要局限于前期排卵卵泡中的 COCs，并且在排卵后 COCs 的颗粒细胞中也存在类似的免疫反应。在前期和排卵后 COCs 中同时存在 AR 和细胞色素 P450c17 免疫标记提示雄激素的内源性和旁分泌调节卵丘颗粒细胞功能。
