School of Engineering, University of Borås, Borås, Sweden.
Bioresour Technol. 2010 Jul;101(13):4914-8. doi: 10.1016/j.biortech.2009.08.100. Epub 2009 Sep 22.
Pretreatment of softwood spruce and hardwood oak with an industrial cellulose solvent, N-methylmorpholine-N-oxide (NMMO), was investigated prior to enzymatic hydrolysis and fermentation to ethanol. The pretreatments were carried out at 90, 110 and 130 degrees C for 1-3 h with 85% NMMO solution, followed by non-isothermal simultaneous saccharification and fermentation (NSSF). This NSSF included hydrolysis with cellulase and beta-glucosidase for 24 h at 45 degrees C, followed by continuous saccharification and fermentation with Saccharomyces cerevisiae at 37 degrees C for 3 days. The NSSF of untreated oak and spruce resulted in 18.6% and 6.8% ethanol compared to the maximum theoretical yield. However, the pretreatment of oak and spruce at 130 degrees C resulted in almost total conversion of cellulose to ethanol and improved ethanol yield up to 85.4% and 89%, respectively. These numbers are comparable with ethanol from pure glucose with the same strain, which yielded between 84% and 90% of the theoretical ethanol yield.
预处理软木云杉和硬木橡木与工业纤维素溶剂 N-甲基吗啉-N-氧化物（NMMO），酶水解和发酵之前进行乙醇。预处理在 90 度，110 和 130 度进行 1-3 小时用 85%NMMO 溶液，随后非等温热同时糖化和发酵（NSSF）。这种 NSSF 包括纤维素酶和β-葡萄糖苷酶在 45 度水解 24 小时，然后在 37 度连续糖化和发酵用酿酒酵母进行 3 天。未经处理的橡木和云杉的 NSSF 与最大理论产率相比产生了 18.6%和 6.8%的乙醇。然而，橡木和云杉在 130 度下的预处理导致纤维素几乎完全转化为乙醇，乙醇产率分别提高到 85.4%和 89%。这些数字与相同菌株的纯葡萄糖的乙醇产量相当，理论乙醇产量为 84%至 90%。
