School of Engineering, University of Borås, Borås, Sweden.
J Ind Microbiol Biotechnol. 2012 Mar;39(3):429-38. doi: 10.1007/s10295-011-1048-y. Epub 2011 Nov 4.
Pretreatment of cellulose with an industrial cellulosic solvent, N-methylmorpholine-N-oxide, showed promising results in increasing the rate of subsequent enzymatic hydrolysis. Cotton linter was used as high crystalline cellulose. After the pretreatment, the cellulose was almost completely hydrolyzed in less than 12 h, using low enzyme loading (15 FPU/g cellulose). The pretreatment significantly decreased the total crystallinity of cellulose from 7.1 to 3.3, and drastically increased the enzyme adsorption capacity of cellulose by approximately 42 times. A semi-mechanistic model was used to describe the relationship between the cellulose concentration and the enzyme loading. In this model, two reactions for heterogeneous reaction of cellulose to glucose and cellobiose, and a homogenous reaction for cellobiose conversion to glucose was incorporated. The Langmuir model was applied to model the adsorption of cellulase onto the treated cellulose. The competitive inhibition was also considered for the effects of sugar inhibition on the rate of enzymatic hydrolysis. The kinetic parameters of the model were estimated by experimental results and evaluated.
预处理纤维素使用工业纤维素溶剂 N-甲基吗啉-N-氧化物，有望提高后续酶水解的速率。棉绒被用作高结晶纤维素。预处理后，使用低酶用量（15 FPU/g 纤维素），纤维素在不到 12 小时内几乎完全水解。预处理显著降低了纤维素的总结晶度，从 7.1 降低到 3.3，并且酶吸附纤维素的能力提高了约 42 倍。使用半机理模型来描述纤维素浓度和酶用量之间的关系。在该模型中，包含了纤维素异相反应生成葡萄糖和纤维二糖的两个反应，以及纤维二糖均相转化生成葡萄糖的反应。采用朗缪尔模型来模拟纤维素酶吸附到处理后的纤维素上。还考虑了糖抑制对酶水解速率的竞争抑制作用。通过实验结果来估计模型的动力学参数，并进行评估。
