Dahlgren U I, Wold A E, Hanson L A, Midtvedt T
Institute of Clinical Immunology, University of Göteborg, Sweden.
Immunology. 1990 Oct;71(2):295-300.
The homing of lymphoid cells to mucosa-associated lymphoid tissue is, amongst other factors, influenced by the nature of the antigen used to induce an immune response. To study this phenomenon we have monocolonized rats with a type 1 fimbriated Escherichia coli O6K13H1 strain and compared the secretory antibody response to colonization with the primary and secondary response obtained in rats immunized in the Peyer's patches (PP). Samples were tested with respect to the titres of antibodies against two antigens present on the E. coli strain: O6 lipopolysaccharide (LPS) and type 1 fimbrial antigen. In the primary immunized animals, IgA anti-fimbrial antibodies were mainly seen in milk and IgA anti-LPS antibodies mostly found in bile. In the booster immunized, and in the monocolonized, animals there was a shift of the antibody response towards the bile. Thus anti-fimbrial antibodies appeared in milk at approximately the same or at a lower level than in bile and the IgA anti-LPS antibodies were almost completely absent in the milk. The IgG antibody response of the animals immunized in the PP was primarily confined to milk for both anti-LPS and anti-fimbrial antibodies, while the colonized animals responded with higher levels in bile than in milk. IgM antibodies were only seen in the milk, except in primary immunized animals in which biliary IgM antibodies also were found. The data illustrate that: (i) primary stimulated cells predestined to produce IgA anti-LPS antibodies home mainly to the intestine, while cells predestined to anti-fimbrial antibody production have a greater tendency to populate the mammary gland; (ii) after repeated antigen stimulation and maturation of the immune response the cells are directed from the mammary gland to the intestine. We thus conclude that the nature of the antigen and the stage of lymphocyte maturation influences the homing of the cells and the appearance of various antibodies in different secretions.
除其他因素外，用于诱导免疫反应的抗原性质会影响淋巴细胞归巢至黏膜相关淋巴组织。为研究这一现象，我们用1型菌毛化大肠杆菌O6K13H1菌株对大鼠进行单菌定植，并将定植后的分泌性抗体反应与在派尔集合淋巴结（PP）免疫的大鼠中获得的初次和二次反应进行比较。针对大肠杆菌菌株上存在的两种抗原的抗体滴度对样本进行了检测：O6脂多糖（LPS）和1型菌毛抗原。在初次免疫的动物中，抗菌毛IgA抗体主要见于乳汁，而抗LPS IgA抗体大多见于胆汁。在加强免疫的动物和单菌定植的动物中，抗体反应向胆汁转移。因此，抗菌毛抗体在乳汁中出现的水平与胆汁中大致相同或低于胆汁，乳汁中几乎完全没有抗LPS IgA抗体。在PP免疫的动物中，IgG抗体反应主要局限于乳汁中的抗LPS和抗菌毛抗体，而定植动物在胆汁中的反应水平高于乳汁。IgM抗体仅见于乳汁，但初次免疫的动物中也发现了胆汁中的IgM抗体。数据表明：（i）注定产生抗LPS IgA抗体的初次刺激细胞主要归巢至肠道，而注定产生抗菌毛抗体的细胞更倾向于在乳腺中聚集；（ii）经过反复抗原刺激和免疫反应成熟后，细胞从乳腺转向肠道。因此，我们得出结论，抗原性质和淋巴细胞成熟阶段会影响细胞归巢以及不同分泌物中各种抗体的出现。
