Allen N, Clendenon N R, Abe H, Swenberg J A, Koestner A, Wechsler W, Shuttleworth E C
Acta Neuropathol. 1977 Jul 15;39(1):13-23. doi: 10.1007/BF00690381.
Nitrosourea induced tumors of the nervous system in rats have proven useful for biochemical studies combined with morphological approaches. The pattern of enzyme activities for acid hydrolases and cytochrome oxidase resemble those previously observed in spontaneous nervous system tumors of man. The activities of 4 acid hydrolases were generally high in the gliomas. This could not be attributed solely to zones of regression or necrosis but was a general characteristic of the neoplasms. The activities were predominantly particulate and most likely lysosomal in localization. In schwannomas a similar increase in hydrolases was found in comparison with normal neural tissues but aryl-sulfatase was not increased. Cytochrome oxidase activities were markedly reduced in all tumors studied. The proportionate reduction with respect to normal brain was comparable to that noted in man. No differences were found with respect to fairly well differentiated gliomas.
已证明，亚硝基脲诱发的大鼠神经系统肿瘤对于结合形态学方法的生化研究很有用。酸性水解酶和细胞色素氧化酶的酶活性模式与先前在人类自发性神经系统肿瘤中观察到的相似。4种酸性水解酶的活性在胶质瘤中通常较高。这不能仅仅归因于退行性变或坏死区域，而是肿瘤的一个普遍特征。这些活性主要存在于颗粒部分，定位上很可能是溶酶体。与正常神经组织相比，在神经鞘瘤中也发现水解酶有类似增加，但芳基硫酸酯酶没有增加。在所研究的所有肿瘤中，细胞色素氧化酶活性均明显降低。相对于正常脑的比例性降低与在人类中观察到的相当。在分化相当良好的胶质瘤方面未发现差异。
