Reinhart H H, Obedeanu N, Sobel J D
Department of Medicine, Wayne State University, Detroit, Michigan.
J Infect Dis. 1990 Dec;162(6):1335-40. doi: 10.1093/infdis/162.6.1335.
In quantitative experiments using ELISA, binding of Tamm-Horsfall protein (THP) to uropathogenic Escherichia coli was studied with monoclonal antibody to THP. Adherence to E. coli bearing type 1 fimbriae was proportional to THP concentration and size of the bacterial inoculum. Type 1 fimbriae-bearing E. coli bound 50 times more THP than did non-type 1-fimbriated or P-fimbriated strains. Concanavalin A and wheat germ agglutinin bound THP in a dose-dependent fashion, whereas pokeweed mitogen and Vicia villosa B4 isolectin did not. Addition of mannose and N-acetylglucosamine reduced adherence of THP to concanavalin A and wheat germ agglutinin by 50%-80%. Sugar inhibition studies suggested that the fimbrial receptor site for THP has lectin-like properties and that THP binds to fimbriae via its mannose side chains. This quantitative assay is useful for studying the interaction between THP, uroepithelial cells, and bacteria in vitro.
在使用酶联免疫吸附测定（ELISA）的定量实验中，用抗Tamm-Horsfall蛋白（THP）的单克隆抗体研究了THP与尿路致病性大肠杆菌的结合情况。与带有1型菌毛的大肠杆菌的黏附与THP浓度及细菌接种量大小成正比。带有1型菌毛的大肠杆菌结合的THP比非1型菌毛或P菌毛菌株多50倍。伴刀豆球蛋白A和麦胚凝集素以剂量依赖方式结合THP，而商陆有丝分裂原和野豌豆B4异凝集素则不然。添加甘露糖和N-乙酰葡糖胺可使THP与伴刀豆球蛋白A和麦胚凝集素的黏附减少50%-80%。糖类抑制研究表明，THP的菌毛受体位点具有凝集素样特性，且THP通过其甘露糖侧链与菌毛结合。这种定量测定法有助于在体外研究THP、尿上皮细胞和细菌之间的相互作用。
