Wu A M, Watkins W M, Chen C P, Song S C, Chow L P, Lin J Y
Glyco-immunochemistry Research Laboratory, Chang-Gung Medical College, Kwei-san, Tao-yuan, Taiwan.
FEBS Lett. 1995 Aug 28;371(1):32-4. doi: 10.1016/0014-5793(95)00862-4.
The binding properties of human Tamm-Horsfall Sd(a+) urinary glycoprotein (THGP) and asialo-THGP with Triticum vulgaris agglutinin(WGA) and three toxic lectins (abrin-a, ricin, and Mistletoe toxic lectin-I) were investigated by quantitative precipitin and precipitin inhibition assays. Both glycoproteins reacted strongly with abrin-a, precipitating over 80% of the lectin nitrogen tested. THGP also bound well to mistletoe toxic lectin-I and precipitated 86% of this lectin added, while the precipitability of its asialo product decreased by 28%. The native glycoprotein completely precipitated the WGA added, but its reactivity was reduced dramatically after desialylation. On the contrary, the poor reactivity of THGP with ricin increased substantially after removal of sialic acid and completely precipitated the lectin added. The glycoprotein-lectin interactions were inhibited by one or several of the following haptens, p-NO2-phenyl alpha GalNAc, p-NO2-phenyl beta GalNAc, Gal beta 1-->4GlcNAc, Gal beta 1-->4Glc, GlcNac beta 1-->4GlcNAc and/or GlcNAc. From the above results, it is concluded that native and/or Tamm-Horsfall glycoproteins serve as important receptors for these three toxic lectins and for WGA.
通过定量沉淀和沉淀抑制试验，研究了人Tamm - Horsfall Sd(a+)尿糖蛋白（THGP）和去唾液酸THGP与小麦凝集素（WGA）以及三种毒性凝集素（相思子毒素-a、蓖麻毒素和槲寄生毒性凝集素-I）的结合特性。两种糖蛋白均与相思子毒素-a强烈反应，沉淀了超过80%的被测凝集素氮。THGP也能很好地结合槲寄生毒性凝集素-I，并沉淀了所添加该凝集素的86%，而其去唾液酸产物的沉淀率下降了28%。天然糖蛋白能完全沉淀所添加的WGA，但其去唾液酸化后反应性显著降低。相反，THGP与蓖麻毒素的反应性较差，但去除唾液酸后反应性大幅增加，并完全沉淀了所添加的凝集素。糖蛋白与凝集素的相互作用受到以下一种或几种半抗原的抑制，即对硝基苯基α - GalNAc、对硝基苯基β - GalNAc、Galβ1→4GlcNAc、Galβ1→4Glc、GlcNacβ1→4GlcNAc和/或GlcNAc。从上述结果得出结论，天然和/或Tamm - Horsfall糖蛋白是这三种毒性凝集素和WGA的重要受体。
