CEA-NeuroSpin, Saclay, France.
Magn Reson Med. 2009 Dec;62(6):1641-5. doi: 10.1002/mrm.22160.
(13)C NMR spectroscopy is a unique tool to measure the cerebral tricarboxylic acid (TCA) cycle rate in vivo. The measurement relies on metabolic modeling of glutamate C3 and C4 enrichment time courses during a (13)C-glucose intravenous infusion. Usual metabolic models require the plasma glucose and (13)C-glucose time courses as input functions, as well as the knowledge of Michaelis-Menten kinetics parameters governing passage through the blood-brain barrier. It is shown in the present work that, when using an infusion protocol yielding a rapidly stable plasma glucose fractional enrichment, metabolic modeling can be simplified in such a manner that this additional information on input function and glucose transport is no longer required, significantly simplifying the measurement of cerebral TCA cycle rate in vivo.
（13）C NMR 光谱是一种独特的工具，可用于测量体内三羧酸（TCA）循环的速率。该测量依赖于（13）C-葡萄糖静脉输注期间谷氨酸 C3 和 C4 富集时程的代谢建模。常用的代谢模型需要将血浆葡萄糖和（13）C-葡萄糖时程作为输入函数，以及控制穿过血脑屏障的米氏动力学参数的知识作为输入函数。本工作表明，当使用产生快速稳定的血浆葡萄糖分数富集的输注方案时，可以简化代谢建模，从而不再需要有关输入函数和葡萄糖转运的这些附加信息，这显著简化了体内三羧酸循环速率的测量。
