Department of Orthopedics, National Taiwan University Hospital Yun-Lin Branch, Douliou City, Yunlin County, Taiwan.
J Orthop Res. 2010 Mar;28(3):379-83. doi: 10.1002/jor.20988.
Nucleus pulposus (NP) cells, sourced from herniation surgeries, may be used as a cell-based therapy for intervertebral disc (IVD) degeneration. But, both the regenerative potential of these degenerative adult NP cells and how to stimulate optimum matrix synthesis is not yet clear. The purpose of the current study was to understand the different phenotypic behaviors between degenerative adult NP cells and normal adolescent NP cells. Degenerative adult NP cells produced a significantly higher amount of proteoglycans and collagens than adolescent cells. Insulin-like growth factor-1 was the only anabolic cytokine with increased endogenous expression in degenerative adult NP cells. TGF-beta1 treatment of degenerative NP cells promoted matrix synthesis but stimulated too much type I collagen and suppressed type II collagen and aggrecan. Adult degenerative NP cells possess upregulated regenerative potential, but stimulation in addition to TGF-beta1 is needed to enhance matrix productivity and optimize the collagen expression profile.
髓核（NP）细胞可从椎间盘突出手术中获取，可作为细胞疗法治疗椎间盘退变。但这些退变的成人 NP 细胞的再生潜能以及如何刺激最佳基质合成尚不清楚。本研究旨在了解退变的成人 NP 细胞与正常青少年 NP 细胞之间的不同表型行为。退变的成人 NP 细胞产生的蛋白聚糖和胶原明显多于青少年细胞。胰岛素样生长因子-1 是唯一一种内源性表达增加的合成代谢细胞因子。转化生长因子-β1（TGF-β1）处理退变 NP 细胞可促进基质合成，但刺激过多的 I 型胶原并抑制 II 型胶原和聚集蛋白聚糖。成人退变 NP 细胞具有上调的再生潜能，但除 TGF-β1 外，还需要其他刺激来提高基质生成能力并优化胶原表达谱。
