Max Planck Institute for Molecular Plant Physiology, Wissenschaftspark Golm, Potsdam-Golm, Germany.
Plant Cell Environ. 2010 Apr;33(4):468-80. doi: 10.1111/j.1365-3040.2009.02047.x. Epub 2009 Sep 23.
Translational genomics, the use of model species to generate knowledge about biological processes and the functions of genes, offers great promise to biotechnologists. Few studies have sought robust responses of model plants to environmental stresses, such as salinity, by altering the stress dosage or by repeating experiments in consecutive years and/or different seasons. We mined our published and unpublished data on legume salt acclimation for robust system features at the ionomic, transcriptomic and metabolomic levels. We analysed data from the model legume Lotus japonicus, obtained through six independent, long-term, non-lethal salt stress experiments which were carried out over two consecutive years. Best possible controlled greenhouse conditions were applied and two main questions asked: how reproducible are results obtained from physiologically meaningful salinity experiments, and what degree of bias may be expected if conclusions are drawn from less well-repeated sampling? A surprisingly large fraction of the transcriptional and metabolic responses to salt stress were not reproducible between experiments. A core set of robust changes was found that was shared between experiments. Many of these robust responses were qualitatively and quantitatively conserved between different accessions of the same species, indicating that the robust responses may be a sound starting point for translational genomics.
转化基因组学利用模式物种来生成关于生物过程和基因功能的知识，为生物技术人员带来了巨大的希望。很少有研究通过改变应激剂量或在连续几年和/或不同季节重复实验，来寻找模式植物对盐度等环境胁迫的稳健响应。我们挖掘了我们已发表和未发表的豆类耐盐数据，以在离子组学、转录组学和代谢组学水平上找到稳健的系统特征。我们分析了模式豆科植物百脉根的实验数据，这些数据来自于两个连续年份的六个独立的、长期的、非致死性盐胁迫实验。应用了尽可能好的可控温室条件，并提出了两个主要问题：从具有生理意义的盐度实验中获得的结果的可重复性如何，如果从重复采样较少的情况下得出结论，可能会出现多大程度的偏差？盐胁迫的转录和代谢反应有很大一部分在实验之间是不可重复的。我们发现了一组稳健的核心变化，这些变化在实验之间是共享的。这些稳健响应中的许多在同一物种的不同品系之间具有定性和定量的一致性，这表明稳健响应可能是转化基因组学的一个良好起点。
