Pan D, Bera A K, Bandyopadhyay S, Das S K, Bandyopadhyay S, Bhattacharyya S, Manna B, De S, Bhattacharya D
Indian Veterinary Research Institute, Eastern Regional Station, Kolkata 700 037, West Bengal, India.
J Helminthol. 2010 Sep;84(3):241-4. doi: 10.1017/S0022149X09990605. Epub 2009 Sep 28.
This article communicates the relative quantification of five isoforms of antigen B (AgB) of Echinococcus granulosus. Relative expression of the AgB was quantified in active and inactive cysts. Cysts with germinal membrane, clear cyst fluid and protoscoleces showed uniform expression of the five isoforms and were utilized as control. Relative expression of AgB1 was the highest in cysts, where calcification has initiated. AgB2 and AgB4 were expressed more in fertile cysts irrespective of the condition of germinal membrane. The lowest expression of AgB3 was seen in calcified cysts. The relative expression of AgB5 could not be correlated with respect to the condition of the cyst because AgB5 is typically expressed by the adult stage of the parasite.
本文传达了细粒棘球绦虫抗原B（AgB）五种同工型的相对定量情况。在活跃和不活跃的囊肿中对AgB的相对表达进行了定量。具有生发膜、清澈囊液和原头节的囊肿显示出五种同工型的均匀表达，并用作对照。AgB1在已开始钙化的囊肿中相对表达最高。无论生发膜状况如何，AgB2和AgB4在可育囊肿中的表达更多。在钙化囊肿中观察到AgB3的表达最低。由于AgB5通常由寄生虫的成虫阶段表达，因此AgB5的相对表达与囊肿状况无关。
