Pan D, Bera A K, De S, Bandyopadhyay S, Das S K, Manna B, Sreevatsava V, Bhattacharya D
Indian Veterinary Research Institute, Eastern Regional Station, 37-Belgachia Road, Kolkata, West Bengal, India.
J Helminthol. 2010 Dec;84(4):394-7. doi: 10.1017/S0022149X10000064. Epub 2010 Feb 15.
The metacestode stage of Echinococcus granulosus is of zoonotic importance. In general, the 14-3-3 protein is involved in multiplication and survival of eukaryotes. Therefore, this communication presents succinct information on relative expression of the 14-3-3 protein in six different morphotypes of cysts of E. granulosus. All isolated E. granulosus belonged to the common sheep strain (G1). Relative expression of the 14-3-3 protein was higher in fertile cysts when compared to sterile cysts. The predicted amino acid sequence of the 14-3-3 protein was closely clustered with zeta-type isoforms 1 and 2 of the 14-3-3 protein. In addition, the present study demonstrates the presence of the 14-3-3 protein which until now had not been detected in the germinal layer. Our findings indicate that the expression of this biomolecule in the germinal layer of sterile organisms may contribute to the development and survival of the parasite in the host. The uniform expression of actin II conclusively proves the survivability of the harvested organisms.
细粒棘球绦虫的中绦期具有人畜共患病重要性。一般来说，14-3-3蛋白参与真核生物的增殖和存活。因此，本通讯提供了关于细粒棘球绦虫六种不同形态类型囊肿中14-3-3蛋白相对表达的简要信息。所有分离出的细粒棘球绦虫均属于常见的绵羊株（G1）。与不育囊肿相比，14-3-3蛋白在可育囊肿中的相对表达更高。14-3-3蛋白的预测氨基酸序列与14-3-3蛋白的ζ型亚型1和2紧密聚类。此外，本研究证明了14-3-3蛋白的存在，此前在生发层中未检测到该蛋白。我们的研究结果表明，这种生物分子在不育生物体生发层中的表达可能有助于寄生虫在宿主体内的发育和存活。肌动蛋白II的均匀表达最终证明了所收获生物体的生存能力。
