Cheng Qi, Zhu Peng, Li Dan, Huang Zhi-Yuan, Liang Hui-Fang, Chen Yi-Fa, Chen Xiao-Ping
Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Zhonghua Wai Ke Za Zhi. 2009 Jun 15;47(12):931-3.
To study the effect of Extractum trametes robiniophila murr on cardiac allograft rejection in mice.
All abdominal heterotopic heart transplantation models were divided into three groups as follows: (A) Extractum trametes robiniophila murr group. (B) Rejection group. (C) Isograft group. In each group, mean survival times (MST) of transplanted hearts and their pathologic histological changes at postoperative fifth day were observed. With fluoroimmunoassay, granzyme B and CD8(+) expressions were examined.
The MST of heart allografts in group A were (6.38 +/- 0.69) d, significantly shorter than that of group B [(8.31 +/- 0.59) d] (P < 0.01). In group A, acute rejection was present in advance; transplanted hearts were seriously damaged into acute rejection pathological grade 3, and CD8(+) T lymphocytes infiltrated diffusely and the expression of granzyme B increased significantly as compared with other groups.
Exclusive application of Extractum trametes robiniophila murr can promote the acute rejection of graft in early phase of postoperation, and the mechanism may be the promoted proliferation and infiltration of CD8(+) T lymphocytes and the increased expression of granzyme B.
研究云芝提取物对小鼠心脏移植排斥反应的影响。
将所有腹部异位心脏移植模型分为以下三组：（A）云芝提取物组。（B）排斥反应组。（C）同基因移植组。观察每组移植心脏的平均存活时间（MST）及其术后第5天的病理组织学变化。采用荧光免疫测定法检测颗粒酶B和CD8(+)的表达。
A组心脏移植的MST为(6.38±0.69)天，明显短于B组(8.31±0.59)天。A组急性排斥反应提前出现；移植心脏严重受损至急性排斥病理3级，与其他组相比，CD8(+)T淋巴细胞弥漫性浸润，颗粒酶B表达明显增加。
单独应用云芝提取物可促进术后早期移植物的急性排斥反应，其机制可能是促进CD8(+)T淋巴细胞的增殖和浸润以及颗粒酶B表达增加。
