[B16黑色素瘤细胞体外诱导骨髓间充质干细胞向内皮细胞分化的初步研究]

[Pilot study of differentiation of bone marrow derived mesenchymal stem cells into endothelial cells induced by B16 melanoma cells in vitro].

作者信息

Ni Chun-Sheng, Zhao Nan, Sun Tao, Zhao Xiu-Lan, Wang Xing-Hui, Gu Qiang, Sun Bao-Cun

机构信息

Department of Pathology, Tianjin Medical University Cancer Hospital, Tianjin 300070, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2009 Jun;38(6):402-7.

PMID:19781348

Abstract

OBJECTIVE

Bone-marrow derived mesenchymal stem cells (BMSC) have the potential to differentiate into endothelial cells. The aim of the study was to investigate the induction process of BMSC by B16 melanoma cells in vitro and to analyze the role of VEGF-a in the process.

METHODS

A co-culture system containing BMSC and B16 melanoma cells based on transwell indirect model was established, and the induction process of BMSC by B16 melanoma cells was studied in vitro.

RESULTS

BMSC were isolated from the bone marrow of C57 mice. BMSC expressed CD105, CD90, CD73, CD44 and CD166, and acquired expressin of endothelial phenotype markers including VEGFR-1, VEGFR-2 and Factor VIII after co-culture with B16 melanoma cells for 48 hours. The expression level of VEGFR-2 would be double and Factor VIII threefold more by extending the co-culture time to 72 hours. In the co-culture system, B16 melanoma cells also up-regulated the expression of VEGF-a.

CONCLUSIONS

VEGF-a plays a significant role in the differentiation of BMSC into cells of endothelial phenotype, therefore, is important to tumor angiogenesis.

摘要

目的

骨髓间充质干细胞（BMSC）具有分化为内皮细胞的潜力。本研究旨在探讨B16黑色素瘤细胞在体外对BMSC的诱导过程，并分析VEGF-a在此过程中的作用。

方法

基于transwell间接模型建立包含BMSC和B16黑色素瘤细胞的共培养体系，体外研究B16黑色素瘤细胞对BMSC的诱导过程。

结果

从C57小鼠骨髓中分离出BMSC。BMSC表达CD105、CD90、CD73、CD44和CD166，与B16黑色素瘤细胞共培养48小时后获得包括VEGFR-1、VEGFR-2和因子VIII在内的内皮表型标志物表达。将共培养时间延长至72小时，VEGFR-2表达水平会加倍，因子VIII增加三倍。在共培养体系中，B16黑色素瘤细胞也上调了VEGF-a的表达。