实验研究应用(99m)Tc 标记环丙沙星在小鼠感染部位的显像。
Experimental studies on imaging of infected site with (99m)Tc-labeled ciprofloxacin in mice.
机构信息
Department of Nuclear Medicine, Second Affiliated Hospital of Sun Yat-Sen University, Guangdong, China.
出版信息
Chin Med J (Engl). 2009 Aug 20;122(16):1907-9.
BACKGROUND
Bacterial infection can pose a substantial diagnostic dilemma. (99m)Tc-labeled ciprofloxacin (CPF) was developed as a biologically active radiopharmaceutical to diagnose infection. In the present research, we studied the biodistribution and imaging properties of infection tracer (99m)Tc-CPF in a mouse model of infection.
METHODS
CPF was labeled with (99m)Tc and the radiochemical purity and labeling rate were measured. A mouse model of infection was established. We then determined the biodistribution of (99m)Tc-CPF and conducted the whole body scintigraphy of the animal model.
RESULTS
(99m)Tc-Ciprotech was stable for at least 6 hours at room temperature. The labeling rate of CPF by (99m)Tc was over 90%. Clearance of radioactivity mainly occurred in the liver and kidney, and the clearance from blood was rapid. Both biodistribution and imaging results showed higher uptake of (99m)Tc-CPF at sites of infection. The infectious tissue/normal tissue ratio peak was 4.30 at 4 hours after injection.
CONCLUSIONS
(99m)Tc-CPF is a sensitive radiopharmaceutical for scintigraphy of infectious lesions and it is easy to prepare.
背景
细菌感染可能构成重大诊断难题。（99m）Tc 标记环丙沙星（CPF）被开发为一种生物活性放射性药物，用于诊断感染。在本研究中，我们研究了感染示踪剂（99m）Tc-CPF 在感染小鼠模型中的生物分布和成像特性。
方法
CPF 用（99m）Tc 标记，测量放射性化学纯度和标记率。建立感染小鼠模型。然后，我们测定了（99m）Tc-CPF 的生物分布，并对动物模型进行了全身闪烁显像。
结果
（99m）Tc-Ciprotech 在室温下至少稳定 6 小时。CPF 的（99m）Tc 标记率超过 90%。放射性清除主要发生在肝脏和肾脏，血液清除迅速。生物分布和成像结果均显示感染部位对（99m）Tc-CPF 的摄取更高。注射后 4 小时感染组织/正常组织比值峰值为 4.30。
结论
（99m）Tc-CPF 是一种用于感染性病变闪烁显像的灵敏放射性药物，易于制备。
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